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Personal profile

Research Interests

G protein-coupled receptors (GPCRs) represent the largest family of cell surface proteins. Agonist binding to GPCRs activates G proteins regulating many cellular effectors. Classically, G protein activation occurs at the plasma membrane and is rapidly terminated by β-arrestin recruitment to the activated GPCRs, promoting G protein uncoupling from receptor, GPCR internalisation and signalling arrest. 

However, recent studies revealed that upon internalisation, some GPCRs continue to activate G proteins from internal compartments leading to sustained production of second messengers far from the plasma membrane. This different spatiotemporal signalling profile allows distinct cellular functions from the ones occurring at the plasma membrane that can be exploited in the near future to design new pharmacological approaches. My research programme aims to tackle important questions underlying this novel signalling mode.

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  • 1 Similar Profiles
G-Protein-Coupled Receptors Medicine & Life Sciences
Angiotensin II Medicine & Life Sciences
Ligands Medicine & Life Sciences
GTP-Binding Proteins Medicine & Life Sciences
Arrestin Medicine & Life Sciences
Dopamine D5 Receptors Medicine & Life Sciences
Angiotensin Type 2 Receptor Medicine & Life Sciences
Arrestins Medicine & Life Sciences

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Projects 2019 2019

Research Output 2004 2018

28 Citations (Scopus)

Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9

Luttrell, L. M., Wang, J., Plouffe, B., Smith, J. S., Yamani, L., Kaur, S., Jean-Charles, P. Y., Gauthier, C., Lee, M. H., Pani, B., Kim, J., Ahn, S., Rajagopal, S., Reiter, E., Bouvier, M., Shenoy, S. K., Laporte, S. A., Rockman, H. A. & Lefkowitz, R. J., 25 Sep 2018, In : Science Signaling. 11, 549, 23 p., eaat7650.

Research output: Contribution to journalArticle

Clustered Regularly Interspaced Short Palindromic Repeats
Arrestins
G-Protein-Coupled Receptors
Small Interfering RNA
GTP-Binding Proteins
6 Citations (Scopus)

Translating biased signaling in the ghrelin receptor system into differential in vivo functions

Mende, F., Hundahl, C., Plouffe, B., Skov, L. J., Sivertsen, B., Madsen, A. N., Lückmann, M., Diep, T. A., Offermanns, S., Frimurer, T. M., Bouvier, M. & Holst, B., 23 Oct 2018, In : Proceedings of the National Academy of Sciences of the United States of America. 115, 43, p. E10255-E10264

Research output: Contribution to journalArticle

Ghrelin Receptor
Ligands
Arrestin
GTP-Binding Proteins
Eating
11 Citations (Scopus)

Type 2 diabetes–associated variants of the MT 2 melatonin receptor affect distinct modes of signaling

Karamitri, A., Plouffe, B., Bonnefond, A., Chen, M., Gallion, J., Guillaume, J. L., Hegron, A., Boissel, M., Canouil, M., Langenberg, C., Wareham, N. J., Le Gouill, C., Lukasheva, V., Lichtarge, O., Froguel, P., Bouvier, M. & Jockers, R., 28 Aug 2018, In : Science Signaling. 11, 545, 14 p., eaan6622.

Research output: Contribution to journalArticle

Melatonin Receptors
Melatonin
Medical problems
Type 2 Diabetes Mellitus
Chemical activation
21 Citations (Scopus)

Discovery of G Protein-Biased Dopaminergics with a Pyrazolo[1,5-a]pyridine Substructure

Möller, D., Banerjee, A., Uzuneser, T. C., Skultety, M., Huth, T., Plouffe, B., Hübner, H., Alzheimer, C., Friedland, K., Müller, C. P., Bouvier, M. & Gmeiner, P., 13 Apr 2017, In : Journal of Medicinal Chemistry. 60, 7, p. 2908-2929 22 p.

Research output: Contribution to journalArticle

Dopamine D2 Receptors
GTP-Binding Proteins
Piperazines
Gi-Go GTP-Binding Protein alpha Subunits
Arrestin
85 Citations (Scopus)

Distinct conformations of GPCR-β-arrestin complexes mediate desensitization, signaling, and endocytosis

Cahill, T. J., Thomsen, A. R. B., Tarrasch, J. T., Plouffe, B., Nguyen, A. H., Yang, F., Huang, L. Y., Kahsai, A. W., Bassoni, D. L., Gavino, B. J., Lamerdin, J. E., Triest, S., Shukla, A. K., Berger, B., Little, J., Antar, A., Blanc, A., Qu, C. X., Chen, X., Kawakami, K. & 7 others, Inoue, A., Aoki, J., Steyaert, J., Sun, J. P., Bouvier, M., Skiniotis, G. & Lefkowitz, R. J., 07 Mar 2017, In : Proceedings of the National Academy of Sciences of the United States of America. 114, 10, p. 2562-2567 6 p.

Research output: Contribution to journalArticle

Arrestin
G-Protein-Coupled Receptors
Endocytosis
Arrestins
GTP-Binding Proteins

Prizes

Best Oral Presentation Award

Bianca Plouffe (Recipient), 01 Sep 2004

Prize: Prize (including medals and awards)

Doctoral Fellowship Award

Bianca Plouffe (Recipient), 01 Sep 2007

Prize: Fellowship awarded competitively

Étudiants-Chercheurs Etoiles Award

Bianca Plouffe (Recipient), 01 Oct 2016

Prize: Prize (including medals and awards)

Excellence Scholarship

Bianca Plouffe (Recipient), 01 Sep 2007

Prize: Prize (including medals and awards)

Graduate/Postdoctoral Travel Award

Bianca Plouffe (Recipient), Apr 2008

Prize: Prize (including medals and awards)

Activities 2004 2019

  • 10 Oral presentation
  • 4 Invited talk

Endosomal G protein signalling by US28 as a new target for glioblastoma multiforme

Bianca Plouffe (Advisor)
23 Oct 2019

Activity: Talk or presentation typesOral presentation

GPCR, G protein and ß-arrestin: three dancers dancing together in the endosome.

Bianca Plouffe (Invited speaker)
04 Jun 2018

Activity: Talk or presentation typesInvited talk

GPCR, G protein and ß-arrestin: three dancers dancing together in the endosome.

Bianca Plouffe (Invited speaker)
24 May 2018

Activity: Talk or presentation typesInvited talk

GPCR, G protein and ß-arrestin: three dancers dancing together in the endosome.

Bianca Plouffe (Invited speaker)
12 Jun 2018

Activity: Talk or presentation typesInvited talk

Human melatonin type 2 receptor structural insights revealed by natural variants.

Bianca Plouffe (Speaker)
26 May 2017

Activity: Talk or presentation typesOral presentation