α-Tocopherol induces proatherogenic changes to HDL2 & HDL3: An in vitro and ex vivo investigation

Lauren Wade, Nida Nadeem, Ian S Young, Jayne V Woodside, Ann McGinty, Cyril McMaster, Jane McEneny

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Introduction: High density lipoproteins (HDL) have considerable potential for improving cardiovascular health. Additionally, epidemiological studies have identified an inverse relationship between a-tocopherol intake and cardiovascular disease, which has not been translated in randomised controlled trials. Objectives: This study assessed if increased α-tocopherol within HDL2 and HDL3 (HDL2&3) influenced their antiatherogenic potential. In the first of two in vitro investigations, the oxidation potential of HDL2&3 was assessed when α-tocopherol was added following their isolation. In the second, their oxidation potential was assessed when HDL2&3 were isolated from serum pre-incubated with α-tocopherol. Additionally, a 6-week placebo-controlled intervention with α-tocopherol assessed if α-tocopherol influenced the oxidation potential and activities of HDL2&3-associated enzymes, paraoxonase-1 (PON-1) and lecithin cholesteryl acyltransferase (LCAT). Results: Conflicting results arose from the in vitro investigations, whereby increasing concentrations of α-tocopherol protected HDL2&3 against oxidation in the post-incubated HDL2&3, and promoted HDL2&3-oxidation when they were isolated from serum pre-incubated with α-tocopherol. Following the 6-week placebo-controlled investigation, α-tocopherol increased in HDL2&3, while HDL2&3 became more susceptible to oxidation, additionally the activities of HDL2&3-PON-1 and HDL2-LCAT decreased. Conclusion: These results have shown for the first time that α-tocopherol induces changes to HDL2&3, which could contribute to the pathophysiology of cardiovascular disease.
Original languageEnglish
Pages (from-to)392-397
Number of pages6
JournalAtherosclerosis
Volume226
Issue number2
Early online date19 Dec 2012
DOIs
Publication statusPublished - Feb 2013

Bibliographical note

Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Keywords

  • Adult
  • Aryldialkylphosphatase
  • Atherosclerosis
  • Female
  • Humans
  • Lipoproteins, HDL
  • Lipoproteins, HDL2
  • Lipoproteins, HDL3
  • Male
  • Oxidation-Reduction
  • Phosphatidylcholine-Sterol O-Acyltransferase
  • Reactive Oxygen Species
  • alpha-Tocopherol

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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