24S,25-Epoxycholesterol is formed in a shunt of the mevalonate pathway that produces cholesterol. It is one of the most potent known activators of the liver X receptors and can inhibit sterol regulatory element-binding protein processing. Until recently analysis of 24S,25-epoxycholesterol at high sensitivity has been precluded by its thermal lability and lack of a strong chromophore. Here we report on the analysis of 24S,25-epoxycholesterol in rodent brain where its level was determined to be of the order of 0.4-1.4μg/g wet weight in both adult mouse and rat. For comparison the level of 24S-hydroxycholesterol in brain of both rodents was of the order of 20μg/g, while that of cholesterol in mouse was 10-20mg/g. By exploiting knockout mice for the enzyme oxysterol 7α-hydroxylase (Cyp7b1) we show that this enzymes is important for the subsequent metabolism of the 24S,25-epoxide.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 27 Jun 2014|
Bibliographical noteCopyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.
- Cholesterol/analogs & derivatives
- Cytochrome P450 Family 7
- Metabolic Networks and Pathways
- Mevalonic Acid/metabolism
- Mice, Congenic
- Mice, Inbred C57BL
- Mice, Knockout
- Models, Biological
- Rats, Sprague-Dawley
- Spectrometry, Mass, Electrospray Ionization
- Steroid Hydroxylases/deficiency