A 2,7-dichlorofluorescein derivative to monitor microcalcifications

Patrik Tholen; Connor N. Brown; Claudia Keil; Ali Bayir; Hui-Hui Zeng; Hajo Haase; Richard B. Thompson; Imre Lengyel; Gündoğ Yücesan

doi:10.1039/d2me00185c

A 2,7-dichlorofluorescein derivative to monitor microcalcifications

Patrik Tholen
, Connor N. Brown
, Claudia Keil
, Ali Bayir
, Hui-Hui Zeng
, Hajo Haase
, Richard B. Thompson
, Imre Lengyel
, Gündoğ Yücesan

Research output: Contribution to journal › Article › peer-review

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Abstract

Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.

Original language	English
Pages (from-to)	1415-1421
Journal	Molecular Systems Design & Engineering
Issue number	11
Early online date	10 Oct 2022
DOIs	https://doi.org/10.1039/d2me00185c
Publication status	Published - 01 Nov 2022

Keywords

Materials Chemistry
Industrial and Manufacturing Engineering
Process Chemistry and Technology
Energy Engineering and Power Technology
Biomedical Engineering
Chemical Engineering (miscellaneous)
Chemistry (miscellaneous)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1039/d2me00185cLicence: CC BY

A 2,7-dichlorofluorescein derivative to monitor microcalcifications
Copyright 2022 the authors. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 1.97 MBLicence: CC BY

Budapest Biannual Calcification meeting
Lengyel, I. (Participant)
01 Oct 2023 → 03 Oct 2023
Activity: Participating in or organising an event types › Participation in conference

Metabolomic changes underpinning the development of sub-retinal pigment epithelial deposits in macular degeneration and Alzheimer's disease.
Brown, C. N. (Author), Lengyel, I. (Supervisor) & Green, B. (Supervisor), Dec 2023
Student thesis: Doctoral Thesis › Doctor of Philosophy

Cite this

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title = "A 2,7-dichlorofluorescein derivative to monitor microcalcifications",

abstract = "Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.",

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T1 - A 2,7-dichlorofluorescein derivative to monitor microcalcifications

AU - Tholen, Patrik

AU - Brown, Connor N.

AU - Keil, Claudia

AU - Bayir, Ali

AU - Zeng, Hui-Hui

AU - Haase, Hajo

AU - Thompson, Richard B.

AU - Lengyel, Imre

AU - Yücesan, Gündoğ

PY - 2022/11/1

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AB - Herein, we report the crystal structure of 2,7-dichlorofluorescein methyl ester (DCF-ME) and its fluorescence response to hydroxyapatite binding. The reported fluorophore is very selective for staining the bone matrix and provides turn-on fluorescence upon hydroxyapatite binding. The reported fluorophore can readily pass the cell membrane of the C2C12 cell line, and it is non-toxic for the cell line. The reported fluorophore DCF-ME may find applications in monitoring bone remodeling and microcalcification as an early diagnosis tool for breast cancer and age-related macular degeneration.

KW - Materials Chemistry

KW - Industrial and Manufacturing Engineering

KW - Process Chemistry and Technology

KW - Energy Engineering and Power Technology

KW - Biomedical Engineering

KW - Chemical Engineering (miscellaneous)

KW - Chemistry (miscellaneous)

U2 - 10.1039/d2me00185c

DO - 10.1039/d2me00185c

M3 - Article

SN - 2058-9689

SP - 1415

EP - 1421

JO - Molecular Systems Design & Engineering

JF - Molecular Systems Design & Engineering

IS - 11

ER -

A 2,7-dichlorofluorescein derivative to monitor microcalcifications

Abstract

Keywords

UN SDGs

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Activities

Budapest Biannual Calcification meeting

Student theses

Metabolomic changes underpinning the development of sub-retinal pigment epithelial deposits in macular degeneration and Alzheimer's disease.

Cite this