A cell surface antigen associated with meiosis in male Staurdoderus scalaris (Orthopteran)

M J Percy, H L Fletcher, A Trudgett

Research output: Contribution to journalArticle

Abstract

Immunofluorescence has identified seven monoclonal antibodies reactive with the surface of meiotic cells and absent in premeiotic cells. Analysis by immunogold electron microscopy indicated that these antigens were present on the external surface of the cells and were coincident with the presence of synaptonemal complexes in the nucleus. On immunoblots a common glycosylated protein of 205 kDa was recognized, in addition to smaller subunits, suggesting the presence of a protein complex comprised of smaller peptides.
Original languageEnglish
Pages (from-to)407-13
Number of pages7
JournalCell Biology International Reports
Volume16
Issue number5
Publication statusPublished - May 1992

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Surface
  • Biological Markers
  • Fluorescent Antibody Technique
  • Grasshoppers
  • Immunohistochemistry
  • Male
  • Meiosis
  • Microscopy, Immunoelectron
  • Spermatozoa

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