A cell surface antigen associated with meiosis in male Staurdoderus scalaris (Orthopteran)

M J Percy; H L Fletcher; A Trudgett

A cell surface antigen associated with meiosis in male Staurdoderus scalaris (Orthopteran)

M J Percy, H L Fletcher, A Trudgett

Research output: Contribution to journal › Article › peer-review

Abstract

Immunofluorescence has identified seven monoclonal antibodies reactive with the surface of meiotic cells and absent in premeiotic cells. Analysis by immunogold electron microscopy indicated that these antigens were present on the external surface of the cells and were coincident with the presence of synaptonemal complexes in the nucleus. On immunoblots a common glycosylated protein of 205 kDa was recognized, in addition to smaller subunits, suggesting the presence of a protein complex comprised of smaller peptides.

Original language	English
Pages (from-to)	407-13
Number of pages	7
Journal	Cell Biology International Reports
Volume	16
Issue number	5
Publication status	Published - May 1992

Keywords

Animals
Antibodies, Monoclonal
Antigens, Surface
Biological Markers
Fluorescent Antibody Technique
Grasshoppers
Immunohistochemistry
Male
Meiosis
Microscopy, Immunoelectron
Spermatozoa

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title = "A cell surface antigen associated with meiosis in male Staurdoderus scalaris (Orthopteran)",

abstract = "Immunofluorescence has identified seven monoclonal antibodies reactive with the surface of meiotic cells and absent in premeiotic cells. Analysis by immunogold electron microscopy indicated that these antigens were present on the external surface of the cells and were coincident with the presence of synaptonemal complexes in the nucleus. On immunoblots a common glycosylated protein of 205 kDa was recognized, in addition to smaller subunits, suggesting the presence of a protein complex comprised of smaller peptides.",

keywords = "Animals, Antibodies, Monoclonal, Antigens, Surface, Biological Markers, Fluorescent Antibody Technique, Grasshoppers, Immunohistochemistry, Male, Meiosis, Microscopy, Immunoelectron, Spermatozoa",

author = "Percy, {M J} and Fletcher, {H L} and A Trudgett",

year = "1992",

month = may,

language = "English",

volume = "16",

pages = "407--13",

journal = "Cell Biology International Reports",

issn = "0309-1651",

publisher = "Academic Press Inc.",

number = "5",

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TY - JOUR

T1 - A cell surface antigen associated with meiosis in male Staurdoderus scalaris (Orthopteran)

AU - Percy, M J

AU - Fletcher, H L

AU - Trudgett, A

PY - 1992/5

Y1 - 1992/5

N2 - Immunofluorescence has identified seven monoclonal antibodies reactive with the surface of meiotic cells and absent in premeiotic cells. Analysis by immunogold electron microscopy indicated that these antigens were present on the external surface of the cells and were coincident with the presence of synaptonemal complexes in the nucleus. On immunoblots a common glycosylated protein of 205 kDa was recognized, in addition to smaller subunits, suggesting the presence of a protein complex comprised of smaller peptides.

AB - Immunofluorescence has identified seven monoclonal antibodies reactive with the surface of meiotic cells and absent in premeiotic cells. Analysis by immunogold electron microscopy indicated that these antigens were present on the external surface of the cells and were coincident with the presence of synaptonemal complexes in the nucleus. On immunoblots a common glycosylated protein of 205 kDa was recognized, in addition to smaller subunits, suggesting the presence of a protein complex comprised of smaller peptides.

KW - Animals

KW - Antibodies, Monoclonal

KW - Antigens, Surface

KW - Biological Markers

KW - Fluorescent Antibody Technique

KW - Grasshoppers

KW - Immunohistochemistry

KW - Male

KW - Meiosis

KW - Microscopy, Immunoelectron

KW - Spermatozoa

M3 - Article

C2 - 1628316

VL - 16

SP - 407

EP - 413

JO - Cell Biology International Reports

JF - Cell Biology International Reports

SN - 0309-1651

IS - 5

ER -

A cell surface antigen associated with meiosis in male Staurdoderus scalaris (Orthopteran)

Abstract

Keywords

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