A chromatin-modifying function of JNK during stem cell differentiation

Vijay K. Tiwari, Michael B. Stadler, Christiane Wirbelauer, Renato Paro, Dirk Schübeler, Christian Beisel

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88 Citations (Scopus)

Abstract

Signaling mediates cellular responses to extracellular stimuli. The c-Jun NH(2)-terminal kinase (JNK) pathway exemplifies one subgroup of the mitogen-activated protein (MAP) kinases, which, besides having established functions in stress response, also contribute to development by an unknown mechanism. We show by genome-wide location analysis that JNK binds to a large set of active promoters during the differentiation of stem cells into neurons. JNK-bound promoters are enriched with binding motifs for the transcription factor NF-Y but not for AP-1. NF-Y occupies these predicted sites, and overexpression of dominant-negative NF-YA reduces the JNK presence on chromatin. We find that histone H3 Ser10 (H3S10) is a substrate for JNK, and JNK-bound promoters are enriched for H3S10 phosphorylation. Inhibition of JNK signaling in post-mitotic neurons reduces phosphorylation at H3S10 and the expression of target genes. These results establish MAP kinase binding and function on chromatin at a novel class of target genes during stem cell differentiation.
Original languageEnglish
Pages (from-to)94-100
Number of pages7
JournalNature Genetics
Volume44
Issue number1
DOIs
Publication statusPublished - Jan 2012

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    Tiwari, V. K., Stadler, M. B., Wirbelauer, C., Paro, R., Schübeler, D., & Beisel, C. (2012). A chromatin-modifying function of JNK during stem cell differentiation. Nature Genetics, 44(1), 94-100. https://doi.org/10.1038/ng.1036