A colloidal nanopatterning and downscaling of a highly periodic au nanoporous EGFET biosensor

Agnes Purwidyantri, Leonardo Kamajaya, Ching Hsiang Chen, Ji Dung Luo, Chiuan Chian Chiou, Ya Chung Tian, Chan Yu Lin, Chia Ming Yang, Chao Sung Lai*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)
13 Downloads (Pure)


The nanopattern of highly ordered and uniform Au nanoporous membranes with different sizes and thicknesses and the downscaling approach through the combination of colloidal based nanosphere lithography (NSL) and thermal evaporation was proposed to fabricate an extending-gate field effect transistor (EGFET) membrane. The fabrication involved the use of PS nanospheres templates of 500 nm and 100 nm in diameters and various Au thickness of 10, 25 and 40 nm. Carried out in the detection of Staphylococcus aureus 16S rRNA hybridization test with analytical range of 101–106 pM DNA targets, the smaller the Au nanoporous diameter made up by the thicker Au layer produced a gradual improvement in potentiometric study. The Au-nanoporous produced by the thickest Au film at 40 nm and smaller diameter of PS nanospheres (100 nm) demonstrated the most optimum threshold voltage shift and limit of detection (LOD) of ∼1 pM altogether with remarkable specificity in the presence of highly concentrated non-specific DNA of other pathogens. Analytical outcomes point out that smaller, periodic and uniform nanoporous EGFET membrane facilitated the larger hybridization signal due to the higher active surface area enabling the more optimum control of DNA orientation and immobilization.

Original languageEnglish
Pages (from-to)H3170-H3177
Number of pages8
JournalJournal of the Electrochemical Society
Issue number4
Publication statusPublished - 28 Feb 2018
Externally publishedYes

Bibliographical note

Funding Information:
The authors wholeheartedly thank the Ministry of Science of Technology of Taiwan for the financial support under contract numbers of MOST 106-2911-1-182-508, MOST 106-2632-E-182-001, and MOST 106-2221-E-182-059-MY3 and Chang Gung Memorial Hospital, Taiwan for the funding support under contract numbers of CMRPD2F0021 and CMRPD3D0112. A great thank is also addressed to Yustina W. Endytia, from the Department of Industrial Design, Chang Gung University for the assistance in sketching work.

Publisher Copyright:
© The Author(s) 2018.

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Renewable Energy, Sustainability and the Environment
  • Surfaces, Coatings and Films
  • Electrochemistry
  • Materials Chemistry


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