A computational approach for peptidomic analysis in taxonomic study of amphibian species

Huiru Zheng*, Piyush C. Ojha, Stephen McClean, Ciaren Graham, Norman D. Black, John G. Hughes, Chris Shaw

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Peptides in the skin secretion of frogs have been studied for some time now because they frequently possess important biological activity such as antibiotic, antimicrobial, or anticancer properties. In this paper, we present a computational approach for measuring the degree of similarity between the entire peptide complement of the skin secretion of specimens from the same or different species. The first step in the analysis is the generation of a mass spectral profile from an experimental high-performance liquid chromatography/electrosparay ionization analysis of the sample. An "overlap" between the mass spectral profiles of different specimens is then proposed as a measure of their similarity. Analysis of specimens from three species of the genus Litoria, viz., L. Aurea, L. Caerulea, and L. Infrafrenata, and Rana Capito of genus Rana shows that the degree of similarity is highest between specimens from the same species, lower for specimens from different species of the same genus, and lowest between specimens from different genera. This indicates that comparison of skin peptide profiles (i.e., mass spectral profiles of skin secretion) is potentially a useful aid in the taxonomic study of amphibian species.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalIEEE Transactions on Nanobioscience
Volume4
Issue number3
DOIs
Publication statusPublished - 01 Sep 2005

Keywords

  • Amphibian
  • Deconvolution
  • Mass spectrometry
  • Pattern analysis
  • Peptide profile
  • Taxonomic study

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science
  • Computer Science Applications
  • Electrical and Electronic Engineering

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