A cross-sectional analysis of cough swabs from non-expectorating CF patients and healthy control participants

Gisli Einarsson, Laura Sherrard, Elizabeth Johnston, Katherine O'Neill, Leanne McIlreavey, Stephanie McGrath, Deirdre Gilpin, Michelle A Murray, Gillian M Lavelle, Noel G. McElvaney, Richard C Boucher, Marianne S Muhlebach, Joseph Elborn, Michael Tunney

Research output: Contribution to journalMeeting abstract

Abstract

Objectives: To determine and compare microbial community structures using cough swabs collected from non-expectorating CF patients and healthy control (HC) participants. Methods: Cough swabs were collected during outpatient appointments at the adult and paediatric CF Centres of the Belfast Health and Social Care Trust (Belfast City Hospital and Royal Belfast Hospital for Sick Children). Healthy, age matched, control (HC) subjects were invited to attend a single study visit by means of e-mail circulation among work colleagues at Queen’s University Belfast and Belfast Health and Social Care Trust. Extended qualitative microbial culture (absence/presence) was performed under aerobic, microaerophilic (5%CO2) and anaerobic conditions at 37°C for 24-72 hrs. Results: Groups (CF vs HC) were well matched for median [range] age (CF, 13 [6-48] years; HC, 15 [6-44] years), gender (female: CF, 56.9%; HC, 42.9%), mean [SD] FEV1 (CF, 86.7 [19.6] pp; HC, 90.2 [11.3] pp) and mean [SD] BMI (CF, 19.9 [4.0] kg/m2; HC, 20.8 [4.7] kg/m2). The median [range] of the cough swab community richness of the CF group (n=50: 7 [0-12]) was greater compared to the HC group (n=30: 6 [0-10]) (P=0.01). Permutational multivariate analysis (Adonis) showed differences in microbial communities between the CF and HC groups (R2=0.035; P=0.01) and between age groups (6-18 vs ≥18 years: R2=0.036; P=0.01) but not gender (R2=0.015; P=0.30). However, the percentage of variation in distances explained by group (3.5%) and age (3.6%) was small. A Bray-Curtis dissimilarity index indicated that there was significant intra-clustering within each of the two groups. Conclusion: Similar to results from previous studies using induced sputum, culture of cough swabs revealed differences in microbial community signatures between CF patients and HC participants. Funding: HSC R&D, Public Health Agency (Northern Ireland), the MRC and the US NIH (HL092964, HL084934, 5R01 HL092964-04) through a US-Ireland Partnership Grant.
Original languageEnglish
Article numberP055
Pages (from-to)S74-S75
Number of pages1
JournalJournal of Cystic Fibrosis
Volume17
Issue numberSupplement 3
Publication statusPublished - 03 Jun 2018
EventEuropean Cystic Fibrosis Conference - Liverpool, United Kingdom
Duration: 06 Jun 201809 Jun 2018

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Cough
Healthy Volunteers
Cross-Sectional Studies
Adonis
Age Groups
Delivery of Health Care
Northern Ireland
Control Groups
Urban Hospitals
Postal Service
Sputum
Ireland
Cluster Analysis
Appointments and Schedules
Outpatients
Multivariate Analysis
Public Health
Pediatrics

Cite this

@article{cac182f35805401eacbf5d8c594f181a,
title = "A cross-sectional analysis of cough swabs from non-expectorating CF patients and healthy control participants",
abstract = "Objectives: To determine and compare microbial community structures using cough swabs collected from non-expectorating CF patients and healthy control (HC) participants. Methods: Cough swabs were collected during outpatient appointments at the adult and paediatric CF Centres of the Belfast Health and Social Care Trust (Belfast City Hospital and Royal Belfast Hospital for Sick Children). Healthy, age matched, control (HC) subjects were invited to attend a single study visit by means of e-mail circulation among work colleagues at Queen’s University Belfast and Belfast Health and Social Care Trust. Extended qualitative microbial culture (absence/presence) was performed under aerobic, microaerophilic (5{\%}CO2) and anaerobic conditions at 37°C for 24-72 hrs. Results: Groups (CF vs HC) were well matched for median [range] age (CF, 13 [6-48] years; HC, 15 [6-44] years), gender (female: CF, 56.9{\%}; HC, 42.9{\%}), mean [SD] FEV1 (CF, 86.7 [19.6] pp; HC, 90.2 [11.3] pp) and mean [SD] BMI (CF, 19.9 [4.0] kg/m2; HC, 20.8 [4.7] kg/m2). The median [range] of the cough swab community richness of the CF group (n=50: 7 [0-12]) was greater compared to the HC group (n=30: 6 [0-10]) (P=0.01). Permutational multivariate analysis (Adonis) showed differences in microbial communities between the CF and HC groups (R2=0.035; P=0.01) and between age groups (6-18 vs ≥18 years: R2=0.036; P=0.01) but not gender (R2=0.015; P=0.30). However, the percentage of variation in distances explained by group (3.5{\%}) and age (3.6{\%}) was small. A Bray-Curtis dissimilarity index indicated that there was significant intra-clustering within each of the two groups. Conclusion: Similar to results from previous studies using induced sputum, culture of cough swabs revealed differences in microbial community signatures between CF patients and HC participants. Funding: HSC R&D, Public Health Agency (Northern Ireland), the MRC and the US NIH (HL092964, HL084934, 5R01 HL092964-04) through a US-Ireland Partnership Grant.",
author = "Gisli Einarsson and Laura Sherrard and Elizabeth Johnston and Katherine O'Neill and Leanne McIlreavey and Stephanie McGrath and Deirdre Gilpin and Murray, {Michelle A} and Lavelle, {Gillian M} and McElvaney, {Noel G.} and Boucher, {Richard C} and Muhlebach, {Marianne S} and Joseph Elborn and Michael Tunney",
year = "2018",
month = "6",
day = "3",
language = "English",
volume = "17",
pages = "S74--S75",
journal = "Journal of Cystic Fibrosis",
issn = "1569-1993",
publisher = "Elsevier Inc.",
number = "Supplement 3",

}

A cross-sectional analysis of cough swabs from non-expectorating CF patients and healthy control participants. / Einarsson, Gisli; Sherrard, Laura; Johnston, Elizabeth; O'Neill, Katherine; McIlreavey, Leanne; McGrath, Stephanie; Gilpin, Deirdre; Murray, Michelle A; Lavelle, Gillian M; McElvaney, Noel G.; Boucher, Richard C; Muhlebach, Marianne S; Elborn, Joseph; Tunney, Michael.

In: Journal of Cystic Fibrosis, Vol. 17, No. Supplement 3, P055, 03.06.2018, p. S74-S75.

Research output: Contribution to journalMeeting abstract

TY - JOUR

T1 - A cross-sectional analysis of cough swabs from non-expectorating CF patients and healthy control participants

AU - Einarsson, Gisli

AU - Sherrard, Laura

AU - Johnston, Elizabeth

AU - O'Neill, Katherine

AU - McIlreavey, Leanne

AU - McGrath, Stephanie

AU - Gilpin, Deirdre

AU - Murray, Michelle A

AU - Lavelle, Gillian M

AU - McElvaney, Noel G.

AU - Boucher, Richard C

AU - Muhlebach, Marianne S

AU - Elborn, Joseph

AU - Tunney, Michael

PY - 2018/6/3

Y1 - 2018/6/3

N2 - Objectives: To determine and compare microbial community structures using cough swabs collected from non-expectorating CF patients and healthy control (HC) participants. Methods: Cough swabs were collected during outpatient appointments at the adult and paediatric CF Centres of the Belfast Health and Social Care Trust (Belfast City Hospital and Royal Belfast Hospital for Sick Children). Healthy, age matched, control (HC) subjects were invited to attend a single study visit by means of e-mail circulation among work colleagues at Queen’s University Belfast and Belfast Health and Social Care Trust. Extended qualitative microbial culture (absence/presence) was performed under aerobic, microaerophilic (5%CO2) and anaerobic conditions at 37°C for 24-72 hrs. Results: Groups (CF vs HC) were well matched for median [range] age (CF, 13 [6-48] years; HC, 15 [6-44] years), gender (female: CF, 56.9%; HC, 42.9%), mean [SD] FEV1 (CF, 86.7 [19.6] pp; HC, 90.2 [11.3] pp) and mean [SD] BMI (CF, 19.9 [4.0] kg/m2; HC, 20.8 [4.7] kg/m2). The median [range] of the cough swab community richness of the CF group (n=50: 7 [0-12]) was greater compared to the HC group (n=30: 6 [0-10]) (P=0.01). Permutational multivariate analysis (Adonis) showed differences in microbial communities between the CF and HC groups (R2=0.035; P=0.01) and between age groups (6-18 vs ≥18 years: R2=0.036; P=0.01) but not gender (R2=0.015; P=0.30). However, the percentage of variation in distances explained by group (3.5%) and age (3.6%) was small. A Bray-Curtis dissimilarity index indicated that there was significant intra-clustering within each of the two groups. Conclusion: Similar to results from previous studies using induced sputum, culture of cough swabs revealed differences in microbial community signatures between CF patients and HC participants. Funding: HSC R&D, Public Health Agency (Northern Ireland), the MRC and the US NIH (HL092964, HL084934, 5R01 HL092964-04) through a US-Ireland Partnership Grant.

AB - Objectives: To determine and compare microbial community structures using cough swabs collected from non-expectorating CF patients and healthy control (HC) participants. Methods: Cough swabs were collected during outpatient appointments at the adult and paediatric CF Centres of the Belfast Health and Social Care Trust (Belfast City Hospital and Royal Belfast Hospital for Sick Children). Healthy, age matched, control (HC) subjects were invited to attend a single study visit by means of e-mail circulation among work colleagues at Queen’s University Belfast and Belfast Health and Social Care Trust. Extended qualitative microbial culture (absence/presence) was performed under aerobic, microaerophilic (5%CO2) and anaerobic conditions at 37°C for 24-72 hrs. Results: Groups (CF vs HC) were well matched for median [range] age (CF, 13 [6-48] years; HC, 15 [6-44] years), gender (female: CF, 56.9%; HC, 42.9%), mean [SD] FEV1 (CF, 86.7 [19.6] pp; HC, 90.2 [11.3] pp) and mean [SD] BMI (CF, 19.9 [4.0] kg/m2; HC, 20.8 [4.7] kg/m2). The median [range] of the cough swab community richness of the CF group (n=50: 7 [0-12]) was greater compared to the HC group (n=30: 6 [0-10]) (P=0.01). Permutational multivariate analysis (Adonis) showed differences in microbial communities between the CF and HC groups (R2=0.035; P=0.01) and between age groups (6-18 vs ≥18 years: R2=0.036; P=0.01) but not gender (R2=0.015; P=0.30). However, the percentage of variation in distances explained by group (3.5%) and age (3.6%) was small. A Bray-Curtis dissimilarity index indicated that there was significant intra-clustering within each of the two groups. Conclusion: Similar to results from previous studies using induced sputum, culture of cough swabs revealed differences in microbial community signatures between CF patients and HC participants. Funding: HSC R&D, Public Health Agency (Northern Ireland), the MRC and the US NIH (HL092964, HL084934, 5R01 HL092964-04) through a US-Ireland Partnership Grant.

M3 - Meeting abstract

VL - 17

SP - S74-S75

JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

SN - 1569-1993

IS - Supplement 3

M1 - P055

ER -