A CUC triplet confers leucine-dependent regulation of the Bacillus subtilis ilv-leu operon

P T Marta, R D Ladner, J A Grandoni

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31 Citations (Scopus)

Abstract

Regulation of the ilv-leu operon probably involves interaction of a tR NA(GAG) with leader region mRNA. Conversion of a CUC (Leu) triplet located within the leader region to UUC (Phe), CGC (Arg), or UAC (Tyr) converted reporter gene expression to control by corresponding amino acids. Conversion of the CUC triplet to CUU (Leu) decreased expression and disrupted regulation. The results suggested that other tRNAs can substitute for tRNA(Leu) but that interactions in addition to pairing of the anticodon with the CUC triplet are important for proper control.

Original languageEnglish
Pages (from-to)2150-3
Number of pages4
JournalJournal of Bacteriology
Volume178
Issue number7
Publication statusPublished - Apr 1996

Keywords

  • Bacillus subtilis
  • Base Sequence
  • Binding Sites
  • Codon
  • Gene Expression Regulation, Bacterial
  • Lac Operon
  • Leucine
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Nucleic Acid Conformation
  • Open Reading Frames
  • Operon
  • RNA, Bacterial
  • RNA, Messenger
  • Structure-Activity Relationship
  • Transcription, Genetic
  • Journal Article

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