A facile carbon dots based fluorescent probe for ultrasensitive detection of ascorbic acid in biological fluids via non-oxidation reduction strategy

Weiheng Kong; Di Wu; Guoliang Li; Xuefeng Chen; Peiwei Gong; Zhiwei Sun; Guang Chen; Lian Xia; Jinmao You; Yongning Wu

doi:10.1016/j.talanta.2017.01.022

A facile carbon dots based fluorescent probe for ultrasensitive detection of ascorbic acid in biological fluids via non-oxidation reduction strategy

Weiheng Kong, Di Wu, Guoliang Li^*, Xuefeng Chen, Peiwei Gong, Zhiwei Sun, Guang Chen, Lian Xia, Jinmao You, Yongning Wu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

A rapid, facile and ultrasensitive fluorescence sensing system based on nitrogen-doped carbon dots (N-doped CDs) for the detection of ascorbic acid (AA) was developed. The highly photoluminescent N-doped CDs with excellent solubility in water and good biocompatibility were prepared by a one-step hydrothermal synthesis and gave the fluorescence quantum yield of 47%. The addition of AA can intensively suppress the fluorescence of the N-doped CDs through the synergistic effect of the inner filter effect (IFE) and the static quenching effect (SQE). Benefited from the remarkable synergistic effect of IFE and SQE, a facile and ultrasensitive sensor was constructed successfully for AA sensing. The detection procedure was achieved within 2 min. The linear response range of AA was obtained from 10⁻³ to 10⁻⁸ M with a detection limit of 5 nM. This developed method enjoyed many merits including more simplicity, lost cost, high sensitivity, good selectivity, rapid response and excellent biocompatibility. Notably, the proposed fluorescent sensor exhibits excellent performance and applicability for AA determination in human serum and rat brain microdialysate, and may provide a fast yet facile route for AA detection in physiological and pathological fields.

Original language	English
Pages (from-to)	677-684
Number of pages	8
Journal	Talanta
Volume	165
Early online date	13 Jan 2017
DOIs	https://doi.org/10.1016/j.talanta.2017.01.022
Publication status	Published - 01 Apr 2017
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (No. 21677085, 21537001, 81472986, 21305076, 21475074 and 21475075), the Project funded by China Postdoctoral Science Foundation (No. 2016M590071), The Natural Science Foundation of Shandong Province (ZR2013BQ019 and ZR2016EMB04) and Project of Shandong Province Higher Educational Science and Technology Program (J16LA03).

Publisher Copyright:
© 2017 Elsevier B.V.

Keywords

Ascorbic acid
Carbon dots
Fluorimetric detection
Inner filter effect

ASJC Scopus subject areas

Analytical Chemistry

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title = "A facile carbon dots based fluorescent probe for ultrasensitive detection of ascorbic acid in biological fluids via non-oxidation reduction strategy",

abstract = "A rapid, facile and ultrasensitive fluorescence sensing system based on nitrogen-doped carbon dots (N-doped CDs) for the detection of ascorbic acid (AA) was developed. The highly photoluminescent N-doped CDs with excellent solubility in water and good biocompatibility were prepared by a one-step hydrothermal synthesis and gave the fluorescence quantum yield of 47%. The addition of AA can intensively suppress the fluorescence of the N-doped CDs through the synergistic effect of the inner filter effect (IFE) and the static quenching effect (SQE). Benefited from the remarkable synergistic effect of IFE and SQE, a facile and ultrasensitive sensor was constructed successfully for AA sensing. The detection procedure was achieved within 2 min. The linear response range of AA was obtained from 10−3 to 10−8 M with a detection limit of 5 nM. This developed method enjoyed many merits including more simplicity, lost cost, high sensitivity, good selectivity, rapid response and excellent biocompatibility. Notably, the proposed fluorescent sensor exhibits excellent performance and applicability for AA determination in human serum and rat brain microdialysate, and may provide a fast yet facile route for AA detection in physiological and pathological fields.",

keywords = "Ascorbic acid, Carbon dots, Fluorimetric detection, Inner filter effect",

author = "Weiheng Kong and Di Wu and Guoliang Li and Xuefeng Chen and Peiwei Gong and Zhiwei Sun and Guang Chen and Lian Xia and Jinmao You and Yongning Wu",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (No. 21677085, 21537001, 81472986, 21305076, 21475074 and 21475075), the Project funded by China Postdoctoral Science Foundation (No. 2016M590071), The Natural Science Foundation of Shandong Province (ZR2013BQ019 and ZR2016EMB04) and Project of Shandong Province Higher Educational Science and Technology Program (J16LA03). Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

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T1 - A facile carbon dots based fluorescent probe for ultrasensitive detection of ascorbic acid in biological fluids via non-oxidation reduction strategy

AU - Kong, Weiheng

AU - Wu, Di

AU - Li, Guoliang

AU - Chen, Xuefeng

AU - Gong, Peiwei

AU - Sun, Zhiwei

AU - Chen, Guang

AU - Xia, Lian

AU - You, Jinmao

AU - Wu, Yongning

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (No. 21677085, 21537001, 81472986, 21305076, 21475074 and 21475075), the Project funded by China Postdoctoral Science Foundation (No. 2016M590071), The Natural Science Foundation of Shandong Province (ZR2013BQ019 and ZR2016EMB04) and Project of Shandong Province Higher Educational Science and Technology Program (J16LA03). Publisher Copyright: © 2017 Elsevier B.V.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - A rapid, facile and ultrasensitive fluorescence sensing system based on nitrogen-doped carbon dots (N-doped CDs) for the detection of ascorbic acid (AA) was developed. The highly photoluminescent N-doped CDs with excellent solubility in water and good biocompatibility were prepared by a one-step hydrothermal synthesis and gave the fluorescence quantum yield of 47%. The addition of AA can intensively suppress the fluorescence of the N-doped CDs through the synergistic effect of the inner filter effect (IFE) and the static quenching effect (SQE). Benefited from the remarkable synergistic effect of IFE and SQE, a facile and ultrasensitive sensor was constructed successfully for AA sensing. The detection procedure was achieved within 2 min. The linear response range of AA was obtained from 10−3 to 10−8 M with a detection limit of 5 nM. This developed method enjoyed many merits including more simplicity, lost cost, high sensitivity, good selectivity, rapid response and excellent biocompatibility. Notably, the proposed fluorescent sensor exhibits excellent performance and applicability for AA determination in human serum and rat brain microdialysate, and may provide a fast yet facile route for AA detection in physiological and pathological fields.

AB - A rapid, facile and ultrasensitive fluorescence sensing system based on nitrogen-doped carbon dots (N-doped CDs) for the detection of ascorbic acid (AA) was developed. The highly photoluminescent N-doped CDs with excellent solubility in water and good biocompatibility were prepared by a one-step hydrothermal synthesis and gave the fluorescence quantum yield of 47%. The addition of AA can intensively suppress the fluorescence of the N-doped CDs through the synergistic effect of the inner filter effect (IFE) and the static quenching effect (SQE). Benefited from the remarkable synergistic effect of IFE and SQE, a facile and ultrasensitive sensor was constructed successfully for AA sensing. The detection procedure was achieved within 2 min. The linear response range of AA was obtained from 10−3 to 10−8 M with a detection limit of 5 nM. This developed method enjoyed many merits including more simplicity, lost cost, high sensitivity, good selectivity, rapid response and excellent biocompatibility. Notably, the proposed fluorescent sensor exhibits excellent performance and applicability for AA determination in human serum and rat brain microdialysate, and may provide a fast yet facile route for AA detection in physiological and pathological fields.

KW - Ascorbic acid

KW - Carbon dots

KW - Fluorimetric detection

KW - Inner filter effect

U2 - 10.1016/j.talanta.2017.01.022

DO - 10.1016/j.talanta.2017.01.022

M3 - Article

C2 - 28153316

AN - SCOPUS:85009223484

SN - 0039-9140

VL - 165

SP - 677

EP - 684

JO - Talanta

JF - Talanta

ER -

A facile carbon dots based fluorescent probe for ultrasensitive detection of ascorbic acid in biological fluids via non-oxidation reduction strategy

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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