A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish

Mònica Campàs, Jaume Reverté, Maria Rambla-Alegre, Katrina Campbell, Arjen Gerssen, Jorge Diogène

Research output: Contribution to journalArticle

Abstract

Tetrodotoxin (TTX) is a potent neurotoxin responsible for many food poisoning incidents and some fatalities. Although mainly associated with the consumption of pufferfish, in recent years, TTX has been found in shellfish, particularly in Europe. In this work, a magnetic bead (MB)-based colorimetric immunoassay was applied to the detection of TTX in Pacific oysters (Crassostrea gigas), razor clams (Solen marginatus) and mussels (Mytilus galloprovincialis). Effective LODs (eLODs) for TTX of 1 μg/kg in oysters and razor clams and 3.3 μg/kg in mussels, significantly below the EFSA guidance threshold (44 μg/kg), were obtained. The strategy was applied to the analysis of naturally-contaminated Pacific oysters (Crassostrea gigas) and mussels (Mytilus edulis) from the Netherlands, and TTX was detected in all samples. The approach, which takes less than 1.5 h, proved to be useful as a rapid and simple method to detect TTX, support shellfish safety and protect consumers.

Original languageEnglish
Pages (from-to)111315
JournalFood & Chemical Toxicology
Volume140
Early online date05 Apr 2020
DOIs
Publication statusPublished - Jun 2020

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