A fluorimeter-based RT-PCR method for the detection and quantitation of porcine cytokines

M. Spagnuolo-Weaver; R. Fuerst; S. T. Campbell; B. M. Meehan; F. McNeilly; B. Adair; G. Allan

doi:10.1016/S0022-1759(99)00114-3

A fluorimeter-based RT-PCR method for the detection and quantitation of porcine cytokines

M. Spagnuolo-Weaver^*
, R. Fuerst
, S. T. Campbell
, B. M. Meehan
, F. McNeilly
, B. Adair
, G. Allan

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

In this paper, we describe a fluorimeter-based, closed-tube, polymerase chain reaction (PCR) assay for the detection and quantification of the mRNA of porcine interleukin 1α (IL1α) and interleukin 2 (IL2) cytokines in peripheral blood leukocytes (PBLs) using melting curve analysis and compare it to a standard PCR performed in a block-based thermocycler. Copyright (C) 1999 Elsevier Science B.V.

Original language	English
Pages (from-to)	19-27
Number of pages	9
Journal	Journal of Immunological Methods
Volume	230
Issue number	1-2
DOIs	https://doi.org/10.1016/S0022-1759(99)00114-3
Publication status	Published - 19 Nov 1999
Externally published	Yes

Bibliographical note

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

Keywords

Cytokines
Fluorimeter-based PCR
PCR
Porcine

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Access to Document

10.1016/S0022-1759(99)00114-3

Cite this

@article{bc9e0b0a43164bb58b4067c4be321401,

title = "A fluorimeter-based RT-PCR method for the detection and quantitation of porcine cytokines",

abstract = "In this paper, we describe a fluorimeter-based, closed-tube, polymerase chain reaction (PCR) assay for the detection and quantification of the mRNA of porcine interleukin 1α (IL1α) and interleukin 2 (IL2) cytokines in peripheral blood leukocytes (PBLs) using melting curve analysis and compare it to a standard PCR performed in a block-based thermocycler. Copyright (C) 1999 Elsevier Science B.V.",

keywords = "Cytokines, Fluorimeter-based PCR, PCR, Porcine",

author = "M. Spagnuolo-Weaver and R. Fuerst and Campbell, \{S. T.\} and Meehan, \{B. M.\} and F. McNeilly and B. Adair and G. Allan",

year = "1999",

month = nov,

day = "19",

doi = "10.1016/S0022-1759(99)00114-3",

language = "English",

volume = "230",

pages = "19--27",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - A fluorimeter-based RT-PCR method for the detection and quantitation of porcine cytokines

AU - Spagnuolo-Weaver, M.

AU - Fuerst, R.

AU - Campbell, S. T.

AU - Meehan, B. M.

AU - McNeilly, F.

AU - Adair, B.

AU - Allan, G.

PY - 1999/11/19

Y1 - 1999/11/19

N2 - In this paper, we describe a fluorimeter-based, closed-tube, polymerase chain reaction (PCR) assay for the detection and quantification of the mRNA of porcine interleukin 1α (IL1α) and interleukin 2 (IL2) cytokines in peripheral blood leukocytes (PBLs) using melting curve analysis and compare it to a standard PCR performed in a block-based thermocycler. Copyright (C) 1999 Elsevier Science B.V.

AB - In this paper, we describe a fluorimeter-based, closed-tube, polymerase chain reaction (PCR) assay for the detection and quantification of the mRNA of porcine interleukin 1α (IL1α) and interleukin 2 (IL2) cytokines in peripheral blood leukocytes (PBLs) using melting curve analysis and compare it to a standard PCR performed in a block-based thermocycler. Copyright (C) 1999 Elsevier Science B.V.

KW - Cytokines

KW - Fluorimeter-based PCR

KW - PCR

KW - Porcine

UR - https://www.scopus.com/pages/publications/0032734430

U2 - 10.1016/S0022-1759(99)00114-3

DO - 10.1016/S0022-1759(99)00114-3

M3 - Article

C2 - 10594350

AN - SCOPUS:0032734430

SN - 0022-1759

VL - 230

SP - 19

EP - 27

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

A fluorimeter-based RT-PCR method for the detection and quantitation of porcine cytokines

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this