A focused ultrasoft X-ray microbeam for targeting cells individually with submicrometer accuracy

M. Folkard, Giuseppe Schettino, B. Vojnovic, S. Gilchrist, A.G. Michette, S.J. Pfauntsch, Kevin Prise, B.D. Michael

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

The application of microbeams is providing new insights into the actions of radiation at the cell and tissue levels. So far, this has been achieved exclusively through the use of collimated charged particles. One alternative is to use ultrasoft X rays, focused by X-ray diffractive optics. We have developed a unique facility that uses 0.2-0.8-mm-diameter zone plates to focus ultrasoft X rays to a beam of less than 1 mum diameter. The zone plate images characteristic K-shell X rays of carbon or aluminum, generated by focusing a beam of 5-10 keV electrons onto the appropriate target. By reflecting the X rays off a grazing-incidence mirror, the contaminating bremsstrahlung radiation is reduced to 2%. The focused X rays are then aimed at selected subcellular targets using rapid automated cell-finding and alignment procedures; up to 3000 cells per hour can be irradiated individually using this arrangement. (C) 2001 by Radiation Research Society.
Original languageEnglish
Pages (from-to)796-804
Number of pages9
JournalRadiation Research
Volume156
Issue number6
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

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    Folkard, M., Schettino, G., Vojnovic, B., Gilchrist, S., Michette, A. G., Pfauntsch, S. J., Prise, K., & Michael, B. D. (2001). A focused ultrasoft X-ray microbeam for targeting cells individually with submicrometer accuracy. Radiation Research, 156(6), 796-804.