A high throughput screen to test the biocompatibility of water-miscible ionic liquids

Martin Rebros; H. Q.Nimal Gunaratne; Jamie Ferguson; Kenneth R. Seddon; Gillian Stephens

doi:10.1039/b815951c

A high throughput screen to test the biocompatibility of water-miscible ionic liquids

Martin Rebros, H. Q.Nimal Gunaratne, Jamie Ferguson, Kenneth R. Seddon, Gillian Stephens^*

^*Corresponding author for this work

Queen's University Belfast

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

The Agar Diffusion test has been used for many years for testing antibiotic susceptibility in clinical laboratories. We wish to report that this simple, rapid test can also be applied to screen ionic liquids for toxicity to microorganisms. We screened 14 water miscible ionic liquids for toxicity for Clostridium butyricum using the test, and compared the results with EC₅₀ values calculated from measurements of growth rates in the presence of the ionic liquids. The Agar Diffusion test could be used reliably to identify ionic liquids which were toxic to C. butyricum, with EC₅₀ values below 0.034 M, and relatively non-toxic structures, with EC₅₀ values above 0.063 M. Therefore, the Agar Diffusion test can be used with confidence to distinguish between toxic and biocompatible ionic liquids.

Original language	English
Pages (from-to)	402-440
Number of pages	39
Journal	Green Chemistry
Volume	11
Issue number	3
DOIs	https://doi.org/10.1039/b815951c
Publication status	Published - 09 Mar 2009

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ASJC Scopus subject areas

Environmental Chemistry
Pollution

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AU - Stephens, Gillian

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AB - The Agar Diffusion test has been used for many years for testing antibiotic susceptibility in clinical laboratories. We wish to report that this simple, rapid test can also be applied to screen ionic liquids for toxicity to microorganisms. We screened 14 water miscible ionic liquids for toxicity for Clostridium butyricum using the test, and compared the results with EC50 values calculated from measurements of growth rates in the presence of the ionic liquids. The Agar Diffusion test could be used reliably to identify ionic liquids which were toxic to C. butyricum, with EC50 values below 0.034 M, and relatively non-toxic structures, with EC50 values above 0.063 M. Therefore, the Agar Diffusion test can be used with confidence to distinguish between toxic and biocompatible ionic liquids.

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A high throughput screen to test the biocompatibility of water-miscible ionic liquids

Abstract

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