A hydrogel based zinc(ii) sensor for use in fluorescent multi-well plate analysis

Graham R C Hamilton; Yingjie Sheng; Bridgeen Callan; Ryan F. Donnelly; John F. Callan

doi:10.1039/c5nj00259a

A hydrogel based zinc(ii) sensor for use in fluorescent multi-well plate analysis

Graham R C Hamilton, Yingjie Sheng, Bridgeen Callan, Ryan F. Donnelly, John F. Callan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article

8 Citations (Scopus)

Abstract

A polymeric hydrogel containing a photoinduced electron transfer (PET) based probe for Zn(ii) has been formulated into the wells of a 96-well plate. Upon addition of Zn(ii) ions to selected wells, the fluorescence of the gel was observed to increase in a concentration dependent manner in the 0.25-1.75 mM range. The millimolar binding constant observed for this probe is higher than that reported for other Zn(ii) probes in the literature and offers the possibility to determine the concentration of this ion in environments where the Zn(ii) concentration is high. The combination of the multi-well plate set-up with fluorescence detection offers the possibility of high-throughput screening using low sample volumes in a timely manner. To the best of our knowledge, this is the first reported example of a polymeric hydrogel sensor for zinc with capability for use in fluorescence multi-well plate assay.

Original language	English
Pages (from-to)	3461-3466
Number of pages	6
Journal	New journal of chemistry
Volume	39
Issue number	5
Early online date	16 Feb 2015
DOIs	https://doi.org/10.1039/c5nj00259a
Publication status	Published - 01 May 2015

ASJC Scopus subject areas

Chemistry(all)
Catalysis
Materials Chemistry

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Hamilton, G. R. C., Sheng, Y., Callan, B., Donnelly, R. F., & Callan, J. F. (2015). A hydrogel based zinc(ii) sensor for use in fluorescent multi-well plate analysis. New journal of chemistry, 39(5), 3461-3466. https://doi.org/10.1039/c5nj00259a

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abstract = "A polymeric hydrogel containing a photoinduced electron transfer (PET) based probe for Zn(ii) has been formulated into the wells of a 96-well plate. Upon addition of Zn(ii) ions to selected wells, the fluorescence of the gel was observed to increase in a concentration dependent manner in the 0.25-1.75 mM range. The millimolar binding constant observed for this probe is higher than that reported for other Zn(ii) probes in the literature and offers the possibility to determine the concentration of this ion in environments where the Zn(ii) concentration is high. The combination of the multi-well plate set-up with fluorescence detection offers the possibility of high-throughput screening using low sample volumes in a timely manner. To the best of our knowledge, this is the first reported example of a polymeric hydrogel sensor for zinc with capability for use in fluorescence multi-well plate assay.",

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