A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities

Bennett S. Lambert, Jean Baptiste Raina, Vicente I. Fernandez, Christian Rinke, Nachshon Siboni, Francesco Rubino, Philip Hugenholtz, Gene W. Tyson, Justin R. Seymour, Roman Stocker*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Microbial interactions influence the productivity and biogeochemistry of the ocean, yet they occur in miniscule volumes that cannot be sampled by traditional oceanographic techniques. To investigate the behaviours of marine microorganisms at spatially relevant scales, we engineered an in situ chemotaxis assay (ISCA) based on microfluidic technology. Here, we describe the fabrication, testing and first field results of the ISCA, demonstrating its value in accessing the microbial behaviours that shape marine ecosystems.

Original languageEnglish
Pages (from-to)1344-1349
Number of pages6
JournalNature Microbiology
Volume2
Issue number10
Early online date28 Aug 2017
DOIs
Publication statusPublished - 01 Oct 2017
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Microbiology (medical)
  • Cell Biology

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