A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities

Bennett S. Lambert; Jean Baptiste Raina; Vicente I. Fernandez; Christian Rinke; Nachshon Siboni; Francesco Rubino; Philip Hugenholtz; Gene W. Tyson; Justin R. Seymour; Roman Stocker

doi:10.1038/s41564-017-0010-9

A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities

Bennett S. Lambert
, Jean Baptiste Raina
, Vicente I. Fernandez
, Christian Rinke
, Nachshon Siboni
, Francesco Rubino
, Philip Hugenholtz
, Gene W. Tyson
, Justin R. Seymour
, Roman Stocker^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

Microbial interactions influence the productivity and biogeochemistry of the ocean, yet they occur in miniscule volumes that cannot be sampled by traditional oceanographic techniques. To investigate the behaviours of marine microorganisms at spatially relevant scales, we engineered an in situ chemotaxis assay (ISCA) based on microfluidic technology. Here, we describe the fabrication, testing and first field results of the ISCA, demonstrating its value in accessing the microbial behaviours that shape marine ecosystems.

Original language	English
Pages (from-to)	1344-1349
Number of pages	6
Journal	Nature Microbiology
Volume	2
Issue number	10
Early online date	28 Aug 2017
DOIs	https://doi.org/10.1038/s41564-017-0010-9
Publication status	Published - 01 Oct 2017
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

ASJC Scopus subject areas

Microbiology
Immunology
Applied Microbiology and Biotechnology
Genetics
Microbiology (medical)
Cell Biology

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Cite this

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AU - Raina, Jean Baptiste

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AU - Rinke, Christian

AU - Siboni, Nachshon

AU - Rubino, Francesco

AU - Hugenholtz, Philip

AU - Tyson, Gene W.

AU - Seymour, Justin R.

AU - Stocker, Roman

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AB - Microbial interactions influence the productivity and biogeochemistry of the ocean, yet they occur in miniscule volumes that cannot be sampled by traditional oceanographic techniques. To investigate the behaviours of marine microorganisms at spatially relevant scales, we engineered an in situ chemotaxis assay (ISCA) based on microfluidic technology. Here, we describe the fabrication, testing and first field results of the ISCA, demonstrating its value in accessing the microbial behaviours that shape marine ecosystems.

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A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities

Abstract

UN SDGs

ASJC Scopus subject areas

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