A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice

Saowalak Adunphatcharaphon; Oluwatobi Kolawole; Thanasat Sooksimuang; Waraporn Panchan; Wannee Wasuthep; Anuwut Petdum; Grit Pichayawaytin; Rungroj Jintamethasawat; Narusorn Doljirapisit; Armote Somboonkaew; Prae Noppakuadrittidej; Sudtida Kaew-Amdee; Manlika Makornwattana; Julie Meneely; Christopher T. Elliott; Awanwee Petchkongkaew; Nitsara Karoonuthaisiri

doi:10.1038/s41538-024-00342-2

A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice

Saowalak Adunphatcharaphon
, Oluwatobi Kolawole
, Thanasat Sooksimuang
, Waraporn Panchan
, Wannee Wasuthep
, Anuwut Petdum
, Grit Pichayawaytin
, Rungroj Jintamethasawat
, Narusorn Doljirapisit
, Armote Somboonkaew
, Prae Noppakuadrittidej
, Sudtida Kaew-Amdee
, Manlika Makornwattana
, Julie Meneely
, Christopher T. Elliott
, Awanwee Petchkongkaew
, Nitsara Karoonuthaisiri^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

28 Downloads (Pure)

Abstract

Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins-aflatoxin B₁, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B₁ in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and quantification of these mycotoxins, with recoveries ranging from 77% to 127%. Detection limits ranged from 0.56 to 1.89 μg/kg, which were well below their regulatory limits, with a relative standard deviation below 25%. This analytical system provides an on-site method for detecting multiple mycotoxins in rice.

Original language	English
Article number	116
Number of pages	12
Journal	npj Science of Food
Volume	8
DOIs	https://doi.org/10.1038/s41538-024-00342-2
Publication status	Published - 31 Dec 2024

Keywords

multiple mycotoxins
food safety
immunoassay
rice

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10.1038/s41538-024-00342-2Licence: CC BY-NC-ND

A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice
Copyright 2024 the authors. This is an open access article published under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited.
Final published version, 2.57 MBLicence: CC BY-NC-ND

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Adunphatcharaphon, S., Kolawole, O., Sooksimuang, T., Panchan, W., Wasuthep, W., Petdum, A., Pichayawaytin, G., Jintamethasawat, R., Doljirapisit, N., Somboonkaew, A., Noppakuadrittidej, P., Kaew-Amdee, S., Makornwattana, M., Meneely, J., Elliott, C. T., Petchkongkaew, A., & Karoonuthaisiri, N. (2024). A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice. npj Science of Food, 8, Article 116. https://doi.org/10.1038/s41538-024-00342-2

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title = "A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice",

abstract = "Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins-aflatoxin B1, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B1 in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and quantification of these mycotoxins, with recoveries ranging from 77\% to 127\%. Detection limits ranged from 0.56 to 1.89 μg/kg, which were well below their regulatory limits, with a relative standard deviation below 25\%. This analytical system provides an on-site method for detecting multiple mycotoxins in rice. ",

keywords = "multiple mycotoxins, food safety, immunoassay, rice",

author = "Saowalak Adunphatcharaphon and Oluwatobi Kolawole and Thanasat Sooksimuang and Waraporn Panchan and Wannee Wasuthep and Anuwut Petdum and Grit Pichayawaytin and Rungroj Jintamethasawat and Narusorn Doljirapisit and Armote Somboonkaew and Prae Noppakuadrittidej and Sudtida Kaew-Amdee and Manlika Makornwattana and Julie Meneely and Elliott, \{Christopher T.\} and Awanwee Petchkongkaew and Nitsara Karoonuthaisiri",

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Adunphatcharaphon, S, Kolawole, O, Sooksimuang, T, Panchan, W, Wasuthep, W, Petdum, A, Pichayawaytin, G, Jintamethasawat, R, Doljirapisit, N, Somboonkaew, A, Noppakuadrittidej, P, Kaew-Amdee, S, Makornwattana, M, Meneely, J, Elliott, CT, Petchkongkaew, A & Karoonuthaisiri, N 2024, 'A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice', npj Science of Food, vol. 8, 116. https://doi.org/10.1038/s41538-024-00342-2

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T1 - A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice

AU - Adunphatcharaphon, Saowalak

AU - Kolawole, Oluwatobi

AU - Sooksimuang, Thanasat

AU - Panchan, Waraporn

AU - Wasuthep, Wannee

AU - Petdum, Anuwut

AU - Pichayawaytin, Grit

AU - Jintamethasawat, Rungroj

AU - Doljirapisit, Narusorn

AU - Somboonkaew, Armote

AU - Noppakuadrittidej, Prae

AU - Kaew-Amdee, Sudtida

AU - Makornwattana, Manlika

AU - Meneely, Julie

AU - Elliott, Christopher T.

AU - Petchkongkaew, Awanwee

AU - Karoonuthaisiri, Nitsara

PY - 2024/12/31

Y1 - 2024/12/31

N2 - Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins-aflatoxin B1, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B1 in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and quantification of these mycotoxins, with recoveries ranging from 77% to 127%. Detection limits ranged from 0.56 to 1.89 μg/kg, which were well below their regulatory limits, with a relative standard deviation below 25%. This analytical system provides an on-site method for detecting multiple mycotoxins in rice.

AB - Co-occurrence of multiple mycotoxins is a growing global food safety concern due to their harmful effects on humans and animals. This study developed an eco-friendly sample preparation method and an innovative multiplex microarray-based lateral flow immunoassay, using a novel portable reader for on-site simultaneous determination of five regulated mycotoxins-aflatoxin B1, T-2 toxin, zearalenone, deoxynivalenol, and fumonisin B1 in rice. The eco-friendly and ultrafast extraction procedure utilizes a bio-based solvent. Principally, the microarray signals generated through a novel luminescent organic dye were captured to quantify mycotoxin levels in samples using a portable reader installed with a user-friendly interface. The assay demonstrates accurate detection and quantification of these mycotoxins, with recoveries ranging from 77% to 127%. Detection limits ranged from 0.56 to 1.89 μg/kg, which were well below their regulatory limits, with a relative standard deviation below 25%. This analytical system provides an on-site method for detecting multiple mycotoxins in rice.

KW - multiple mycotoxins

KW - food safety

KW - immunoassay

KW - rice

U2 - 10.1038/s41538-024-00342-2

DO - 10.1038/s41538-024-00342-2

M3 - Article

SN - 2396-8370

VL - 8

JO - npj Science of Food

JF - npj Science of Food

M1 - 116

ER -

A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice

Abstract

Keywords

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