A narrow-band ultraviolet B course improves vitamin D balance and alters cutaneous CYP27A1 and CYP27B1 mRNA expression levels in haemodialysis patients supplemented with oral vitamin D

Meri J Ala-Houhala; Katja Vähävihu; Erna Snellman; Taina Hasan; Hannu Kautiainen; Piia Karisola; Yvonne Dombrowski; Jürgen Schauber; Heikki Saha; Timo Reunala

doi:10.1159/000355010

A narrow-band ultraviolet B course improves vitamin D balance and alters cutaneous CYP27A1 and CYP27B1 mRNA expression levels in haemodialysis patients supplemented with oral vitamin D

Meri J Ala-Houhala, Katja Vähävihu, Erna Snellman, Taina Hasan, Hannu Kautiainen, Piia Karisola, Yvonne Dombrowski, Jürgen Schauber, Heikki Saha, Timo Reunala

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Background: Chronic kidney disease (CKD) patients on dialysis are prone to vitamin D insufficiency despite oral vitamin D supplementation. Here, we studied whether narrow-band ultraviolet B (NB-UVB) exposures improve vitamin D balance.

Methods: 14 haemodialysis patients and 15 healthy subjects receiving oral cholecalciferol 20 µg daily got nine NB-UVB exposures on the entire body. Serum 25-hydroxyvitamin D (25(OH)D) was measured by radioimmunoassay. Cutaneous mRNA expression levels of CYP27A1 and CYP27B1, two enzymes required for hydroxylation of vitamin D into its active metabolite, were also measured.

Results: The baseline serum 25(OH)D concentration was 57.6 ± 18.2 nmol/l in the CKD patients and 74.3 ± 14.8 nmol/l in the healthy subjects. The NB-UVB course increased serum 25(OH)D by 14.0 nmol/l (95% CI 8.7-19.5) and 17.0 nmol/l (CI 13.7-20.2), respectively. At baseline the CKD patients showed significantly increased CYP27B1 levels compared to the healthy subjects.

Conclusions: A short NB-UVB course is an efficient way to improve vitamin D balance in CKD patients on dialysis who are receiving oral vitamin D supplementation. The increased cutaneous CYP27B1 levels in the CKD patients suggest that the loss of renal activity of this enzyme is at least partially compensated for by the skin.

Original language	English
Pages (from-to)	17-22
Number of pages	6
Journal	Nephron Clinical Practice
Volume	124
Issue number	1-2
DOIs	https://doi.org/10.1159/000355010
Publication status	Published - Dec 2013

Bibliographical note

Keywords

25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Administration, Oral
Adolescent
Aged
Cholestanetriol 26-Monooxygenase
Combined Modality Therapy
Dietary Supplements
Female
Humans
Male
RNA, Messenger
Renal Dialysis
Renal Insufficiency, Chronic
Skin
Steroid Hydroxylases
Treatment Outcome
Ultraviolet Therapy
Vitamin D
Vitamin D Deficiency
Young Adult

Access to Document

10.1159/000355010

Fingerprint Dive into the research topics of 'A narrow-band ultraviolet B course improves vitamin D balance and alters cutaneous CYP27A1 and CYP27B1 mRNA expression levels in haemodialysis patients supplemented with oral vitamin D'. Together they form a unique fingerprint.

Cite this

Ala-Houhala, M. J., Vähävihu, K., Snellman, E., Hasan, T., Kautiainen, H., Karisola, P., Dombrowski, Y., Schauber, J., Saha, H., & Reunala, T. (2013). A narrow-band ultraviolet B course improves vitamin D balance and alters cutaneous CYP27A1 and CYP27B1 mRNA expression levels in haemodialysis patients supplemented with oral vitamin D. Nephron Clinical Practice, 124(1-2), 17-22. https://doi.org/10.1159/000355010

Ala-Houhala, Meri J ; Vähävihu, Katja ; Snellman, Erna ; Hasan, Taina ; Kautiainen, Hannu ; Karisola, Piia ; Dombrowski, Yvonne ; Schauber, Jürgen ; Saha, Heikki ; Reunala, Timo. / A narrow-band ultraviolet B course improves vitamin D balance and alters cutaneous CYP27A1 and CYP27B1 mRNA expression levels in haemodialysis patients supplemented with oral vitamin D. In: Nephron Clinical Practice. 2013 ; Vol. 124, No. 1-2. pp. 17-22.

@article{ec5d2529f4ce4163ad6bc824f9e6aa76,

title = "A narrow-band ultraviolet B course improves vitamin D balance and alters cutaneous CYP27A1 and CYP27B1 mRNA expression levels in haemodialysis patients supplemented with oral vitamin D",

abstract = "Background: Chronic kidney disease (CKD) patients on dialysis are prone to vitamin D insufficiency despite oral vitamin D supplementation. Here, we studied whether narrow-band ultraviolet B (NB-UVB) exposures improve vitamin D balance.Methods: 14 haemodialysis patients and 15 healthy subjects receiving oral cholecalciferol 20 µg daily got nine NB-UVB exposures on the entire body. Serum 25-hydroxyvitamin D (25(OH)D) was measured by radioimmunoassay. Cutaneous mRNA expression levels of CYP27A1 and CYP27B1, two enzymes required for hydroxylation of vitamin D into its active metabolite, were also measured. Results: The baseline serum 25(OH)D concentration was 57.6 ± 18.2 nmol/l in the CKD patients and 74.3 ± 14.8 nmol/l in the healthy subjects. The NB-UVB course increased serum 25(OH)D by 14.0 nmol/l (95% CI 8.7-19.5) and 17.0 nmol/l (CI 13.7-20.2), respectively. At baseline the CKD patients showed significantly increased CYP27B1 levels compared to the healthy subjects. Conclusions: A short NB-UVB course is an efficient way to improve vitamin D balance in CKD patients on dialysis who are receiving oral vitamin D supplementation. The increased cutaneous CYP27B1 levels in the CKD patients suggest that the loss of renal activity of this enzyme is at least partially compensated for by the skin.",

keywords = "25-Hydroxyvitamin D3 1-alpha-Hydroxylase, Administration, Oral, Adolescent, Aged, Cholestanetriol 26-Monooxygenase, Combined Modality Therapy, Dietary Supplements, Female, Humans, Male, RNA, Messenger, Renal Dialysis, Renal Insufficiency, Chronic, Skin, Steroid Hydroxylases, Treatment Outcome, Ultraviolet Therapy, Vitamin D, Vitamin D Deficiency, Young Adult",

author = "Ala-Houhala, {Meri J} and Katja V{\"a}h{\"a}vihu and Erna Snellman and Taina Hasan and Hannu Kautiainen and Piia Karisola and Yvonne Dombrowski and J{\"u}rgen Schauber and Heikki Saha and Timo Reunala",

note = "Copyright {\textcopyright} 2013 S. Karger AG, Basel.",

year = "2013",

month = dec,

doi = "10.1159/000355010",

language = "English",

volume = "124",

pages = "17--22",

journal = "Nephron Clinical Practice",

issn = "1660-2110",

publisher = "S. Karger AG",

number = "1-2",

}

Ala-Houhala, MJ, Vähävihu, K, Snellman, E, Hasan, T, Kautiainen, H, Karisola, P, Dombrowski, Y, Schauber, J, Saha, H & Reunala, T 2013, 'A narrow-band ultraviolet B course improves vitamin D balance and alters cutaneous CYP27A1 and CYP27B1 mRNA expression levels in haemodialysis patients supplemented with oral vitamin D', Nephron Clinical Practice, vol. 124, no. 1-2, pp. 17-22. https://doi.org/10.1159/000355010

A narrow-band ultraviolet B course improves vitamin D balance and alters cutaneous CYP27A1 and CYP27B1 mRNA expression levels in haemodialysis patients supplemented with oral vitamin D. / Ala-Houhala, Meri J; Vähävihu, Katja; Snellman, Erna; Hasan, Taina; Kautiainen, Hannu; Karisola, Piia; Dombrowski, Yvonne; Schauber, Jürgen; Saha, Heikki; Reunala, Timo.

In: Nephron Clinical Practice, Vol. 124, No. 1-2, 12.2013, p. 17-22.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A narrow-band ultraviolet B course improves vitamin D balance and alters cutaneous CYP27A1 and CYP27B1 mRNA expression levels in haemodialysis patients supplemented with oral vitamin D

AU - Ala-Houhala, Meri J

AU - Vähävihu, Katja

AU - Snellman, Erna

AU - Hasan, Taina

AU - Kautiainen, Hannu

AU - Karisola, Piia

AU - Dombrowski, Yvonne

AU - Schauber, Jürgen

AU - Saha, Heikki

AU - Reunala, Timo

PY - 2013/12

Y1 - 2013/12

N2 - Background: Chronic kidney disease (CKD) patients on dialysis are prone to vitamin D insufficiency despite oral vitamin D supplementation. Here, we studied whether narrow-band ultraviolet B (NB-UVB) exposures improve vitamin D balance.Methods: 14 haemodialysis patients and 15 healthy subjects receiving oral cholecalciferol 20 µg daily got nine NB-UVB exposures on the entire body. Serum 25-hydroxyvitamin D (25(OH)D) was measured by radioimmunoassay. Cutaneous mRNA expression levels of CYP27A1 and CYP27B1, two enzymes required for hydroxylation of vitamin D into its active metabolite, were also measured. Results: The baseline serum 25(OH)D concentration was 57.6 ± 18.2 nmol/l in the CKD patients and 74.3 ± 14.8 nmol/l in the healthy subjects. The NB-UVB course increased serum 25(OH)D by 14.0 nmol/l (95% CI 8.7-19.5) and 17.0 nmol/l (CI 13.7-20.2), respectively. At baseline the CKD patients showed significantly increased CYP27B1 levels compared to the healthy subjects. Conclusions: A short NB-UVB course is an efficient way to improve vitamin D balance in CKD patients on dialysis who are receiving oral vitamin D supplementation. The increased cutaneous CYP27B1 levels in the CKD patients suggest that the loss of renal activity of this enzyme is at least partially compensated for by the skin.

AB - Background: Chronic kidney disease (CKD) patients on dialysis are prone to vitamin D insufficiency despite oral vitamin D supplementation. Here, we studied whether narrow-band ultraviolet B (NB-UVB) exposures improve vitamin D balance.Methods: 14 haemodialysis patients and 15 healthy subjects receiving oral cholecalciferol 20 µg daily got nine NB-UVB exposures on the entire body. Serum 25-hydroxyvitamin D (25(OH)D) was measured by radioimmunoassay. Cutaneous mRNA expression levels of CYP27A1 and CYP27B1, two enzymes required for hydroxylation of vitamin D into its active metabolite, were also measured. Results: The baseline serum 25(OH)D concentration was 57.6 ± 18.2 nmol/l in the CKD patients and 74.3 ± 14.8 nmol/l in the healthy subjects. The NB-UVB course increased serum 25(OH)D by 14.0 nmol/l (95% CI 8.7-19.5) and 17.0 nmol/l (CI 13.7-20.2), respectively. At baseline the CKD patients showed significantly increased CYP27B1 levels compared to the healthy subjects. Conclusions: A short NB-UVB course is an efficient way to improve vitamin D balance in CKD patients on dialysis who are receiving oral vitamin D supplementation. The increased cutaneous CYP27B1 levels in the CKD patients suggest that the loss of renal activity of this enzyme is at least partially compensated for by the skin.

KW - 25-Hydroxyvitamin D3 1-alpha-Hydroxylase

KW - Administration, Oral

KW - Adolescent

KW - Aged

KW - Cholestanetriol 26-Monooxygenase

KW - Combined Modality Therapy

KW - Dietary Supplements

KW - Female

KW - Humans

KW - Male

KW - RNA, Messenger

KW - Renal Dialysis

KW - Renal Insufficiency, Chronic

KW - Skin

KW - Steroid Hydroxylases

KW - Treatment Outcome

KW - Ultraviolet Therapy

KW - Vitamin D

KW - Vitamin D Deficiency

KW - Young Adult

U2 - 10.1159/000355010

DO - 10.1159/000355010

M3 - Article

C2 - 24029861

VL - 124

SP - 17

EP - 22

JO - Nephron Clinical Practice

JF - Nephron Clinical Practice

SN - 1660-2110

IS - 1-2

ER -