A novel antimicrobial peptide, Dermaseptin-SS1, with anti-proliferative activity, isolated from the skin secretion of Phyllomedusa tarsius

Xiaonan Ma, Yuping Chen, Anmei Shu, Yangyang Jiang*, Xiaoling Chen, Chengbang Ma, Mei Zhou, Tao Wang*, Tianbao Chen, Chris Shaw, Lei Wang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

The emergence of multidrug-resistant bacteria has severely increased the burden on the global health system, and such pathogenic infections are considered a great threat to human well-being. Antimicrobial peptides, due to their potent antimicrobial activity and low possibility of inducing resistance, are increasingly attracting great interest. Herein, a novel dermaseptin peptide, named Dermaseptin-SS1 (SS1), was identified from a skin-secretion-derived cDNA library of the South/Central American tarsier leaf frog, Phyllomedusa tarsius, using a ‘shotgun’ cloning strategy. The chemically synthesized peptide SS1 was found to be broadly effective against Gram-negative bacteria with low haemolytic activity in vitro. A designed synthetic analogue of SS1, named peptide 14V5K, showed lower salt sensitivity and more rapid bacteria killing compared to SS1. Both peptides employed a membrane-targeting mechanism to kill Escherichia coli. The antiproliferative activity of SS1 and its analogues against lung cancer cell lines was found to be significant.

Original languageEnglish
Article number6558
Number of pages20
JournalMolecules
Volume28
Issue number18
DOIs
Publication statusPublished - 11 Sept 2023

Keywords

  • antimicrobial peptide
  • dermaseptin
  • molecular cloning
  • analogue design
  • Analytical Chemistry
  • Pharmaceutical Science
  • Molecular Medicine
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Chemistry (miscellaneous)
  • Organic Chemistry

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