Amphibian skin secretions are remarkable sources of novel bioactive peptides. Among these, antimicrobial peptides have demonstrated an outstanding efficacy in killing microorganisms via a general membranolytic mechanism, which may offer the prospect of solving specific target-driven antibiotic resistance. Here, the discovery of a novel defensive peptide is described from the skin secretion of the African frog, Kassina senegalensis. Named kassinatuerin-3, it was identified through a combination of “shot-gun” cloning and MS/MS fragmentation sequencing. Subsequently, a synthetic replicate was subjected to biofunctional evaluation. The results indicated that kassinatuerin-3 possessed antimicrobial activity against Gram-positive bacteria but no effect against Gram-negative bacteria. Additionally, it was active in biofilm eradication on S. aureus and MRSA and in the antiproliferation of selected cancer cell lines. Moreover, it had a very mild hemolytic effect, which demonstrated a high therapeutic index for kassinatuerin-3. Collectively, although kassinatuerin-3 did not demonstrate remarkable bioactivities compared with other natural or synthetic antimicrobial peptides (AMPs), it offered a new insight into the design of antimicrobial derivatives.
|Number of pages||14|
|Early online date||02 Jul 2020|
|Publication status||Early online date - 02 Jul 2020|
- amphibian skin secretion; Kassina senegalensis; antimicrobial peptide; antibiofilm; molecular cloning
FingerprintDive into the research topics of 'A Novel Antimicrobial Peptide (Kassinatuerin-3) Isolated from the Skin Secretion of the African Frog, Kassina senegalensis'. Together they form a unique fingerprint.
Discovery, structural characterisation and targeted engineering of bioactive peptides from amphibian skin secretionAuthor: He, H., Dec 2020
Supervisor: Zhou, M. (Supervisor), Wang, L. (Supervisor), Xi, X. (Supervisor) & Chen, T. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy