A novel antimicrobial peptide, Ranatuerin-2PLx (R2PLx), showing therapeutic potential in inhibiting proliferation of cancer cells

Xiaoling Chen, Luyao Zhang, Chengbang Ma, Yingqi Zhang, Xinping Xi, Lei Wang, Mei Zhou, James F. Burrows, Tianbao Chen

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)
293 Downloads (Pure)

Abstract

Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, a 28-mer polypeptide, R2PLx, was identified from lyophilised skin secretions. The chemically-synthetic replicates exhibited moderate and broad-spectrum antimicrobial effect against various microorganisms including methicillin-resistant S. aureus (MRSA, MIC=256 μM). In additional, R2PLx was found to inhibit the proliferation of several tumour cells, especially showing more potent effect on prostate cancer cell, PC-3. The early cell apoptosis was observed in 6 h by Annexin V-FITC/PI staining, as well as the activation of Caspase-3 at 5 μM peptide concentration. R2PLx may therefore be promising for developing new therapeutic approach for cancer treatment. In addition, the artificial deficiency of conserved rana-box loop or net positive charge in C-terminal domain notably reduced the biological activities of the truncated and substituted isoforms, respectively, suggesting for maintaining their biological potency of Ranatuerin family requires both cysteine-bridged segment and cationincity within the loop domain in C-terminus.
Original languageEnglish
Article numberBSR20180710
Number of pages11
JournalBioscience Reports
Volume38
Issue number6
Early online date02 Oct 2018
DOIs
Publication statusPublished - Dec 2018

Keywords

  • anuran skin secretions, peptides, antibacterial properties, anti-cancer, rana-box

Fingerprint

Dive into the research topics of 'A novel antimicrobial peptide, Ranatuerin-2PLx (R2PLx), showing therapeutic potential in inhibiting proliferation of cancer cells'. Together they form a unique fingerprint.

Cite this