Abstract
| Language | English |
|---|---|
| Article number | BSR20180710 |
| Number of pages | 11 |
| Journal | Bioscience Reports |
| Volume | 38 |
| Issue number | 6 |
| Early online date | 02 Oct 2018 |
| DOIs | |
| Publication status | Published - Dec 2018 |
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Keywords
- anuran skin secretions, peptides, antibacterial properties, anti-cancer, rana-box
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A novel antimicrobial peptide, Ranatuerin-2PLx (R2PLx), showing therapeutic potential in inhibiting proliferation of cancer cells. / Chen, Xiaoling; Zhang, Luyao; Ma, Chengbang; Zhang, Yingqi; Xi, Xinping; Wang, Lei; Zhou, Mei; Burrows, James F.; Chen, Tianbao.
In: Bioscience Reports, Vol. 38, No. 6, BSR20180710, 12.2018.Research output: Contribution to journal › Article
TY - JOUR
T1 - A novel antimicrobial peptide, Ranatuerin-2PLx (R2PLx), showing therapeutic potential in inhibiting proliferation of cancer cells
AU - Chen, Xiaoling
AU - Zhang, Luyao
AU - Ma, Chengbang
AU - Zhang, Yingqi
AU - Xi, Xinping
AU - Wang, Lei
AU - Zhou, Mei
AU - Burrows, James F.
AU - Chen, Tianbao
PY - 2018/12
Y1 - 2018/12
N2 - Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, a 28-mer polypeptide, R2PLx, was identified from lyophilised skin secretions. The chemically-synthetic replicates exhibited moderate and broad-spectrum antimicrobial effect against various microorganisms including methicillin-resistant S. aureus (MRSA, MIC=256 μM). In additional, R2PLx was found to inhibit the proliferation of several tumour cells, especially showing more potent effect on prostate cancer cell, PC-3. The early cell apoptosis was observed in 6 h by Annexin V-FITC/PI staining, as well as the activation of Caspase-3 at 5 μM peptide concentration. R2PLx may therefore be promising for developing new therapeutic approach for cancer treatment. In addition, the artificial deficiency of conserved rana-box loop or net positive charge in C-terminal domain notably reduced the biological activities of the truncated and substituted isoforms, respectively, suggesting for maintaining their biological potency of Ranatuerin family requires both cysteine-bridged segment and cationincity within the loop domain in C-terminus.
AB - Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, a 28-mer polypeptide, R2PLx, was identified from lyophilised skin secretions. The chemically-synthetic replicates exhibited moderate and broad-spectrum antimicrobial effect against various microorganisms including methicillin-resistant S. aureus (MRSA, MIC=256 μM). In additional, R2PLx was found to inhibit the proliferation of several tumour cells, especially showing more potent effect on prostate cancer cell, PC-3. The early cell apoptosis was observed in 6 h by Annexin V-FITC/PI staining, as well as the activation of Caspase-3 at 5 μM peptide concentration. R2PLx may therefore be promising for developing new therapeutic approach for cancer treatment. In addition, the artificial deficiency of conserved rana-box loop or net positive charge in C-terminal domain notably reduced the biological activities of the truncated and substituted isoforms, respectively, suggesting for maintaining their biological potency of Ranatuerin family requires both cysteine-bridged segment and cationincity within the loop domain in C-terminus.
KW - anuran skin secretions, peptides, antibacterial properties, anti-cancer, rana-box
U2 - 10.1042/BSR20180710
DO - 10.1042/BSR20180710
M3 - Article
VL - 38
JO - Bioscience Reports
T2 - Bioscience Reports
JF - Bioscience Reports
SN - 0144-8463
IS - 6
M1 - BSR20180710
ER -