A novel antimicrobial peptide, Ranatuerin-2PLx (R2PLx), showing therapeutic potential in inhibiting proliferation of cancer cells

Xiaoling Chen, Luyao Zhang, Chengbang Ma, Yingqi Zhang, Xinping Xi, Lei Wang, Mei Zhou, James F. Burrows, Tianbao Chen

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2 Citations (Scopus)

Abstract

Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, a 28-mer polypeptide, R2PLx, was identified from lyophilised skin secretions. The chemically-synthetic replicates exhibited moderate and broad-spectrum antimicrobial effect against various microorganisms including methicillin-resistant S. aureus (MRSA, MIC=256 μM). In additional, R2PLx was found to inhibit the proliferation of several tumour cells, especially showing more potent effect on prostate cancer cell, PC-3. The early cell apoptosis was observed in 6 h by Annexin V-FITC/PI staining, as well as the activation of Caspase-3 at 5 μM peptide concentration. R2PLx may therefore be promising for developing new therapeutic approach for cancer treatment. In addition, the artificial deficiency of conserved rana-box loop or net positive charge in C-terminal domain notably reduced the biological activities of the truncated and substituted isoforms, respectively, suggesting for maintaining their biological potency of Ranatuerin family requires both cysteine-bridged segment and cationincity within the loop domain in C-terminus.
LanguageEnglish
Article numberBSR20180710
Number of pages11
JournalBioscience Reports
Volume38
Issue number6
Early online date02 Oct 2018
DOIs
Publication statusPublished - Dec 2018

Fingerprint

Cells
Cell Proliferation
Peptides
Neoplasms
Ranidae
Methicillin Resistance
Methicillin
Oncology
Fluorescein-5-isothiocyanate
Annexin A5
Therapeutics
Methicillin-Resistant Staphylococcus aureus
Bioactivity
Caspase 3
Microorganisms
Cysteine
Tumors
Prostatic Neoplasms
Skin
Protein Isoforms

Keywords

  • anuran skin secretions, peptides, antibacterial properties, anti-cancer, rana-box

Cite this

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title = "A novel antimicrobial peptide, Ranatuerin-2PLx (R2PLx), showing therapeutic potential in inhibiting proliferation of cancer cells",
abstract = "Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, a 28-mer polypeptide, R2PLx, was identified from lyophilised skin secretions. The chemically-synthetic replicates exhibited moderate and broad-spectrum antimicrobial effect against various microorganisms including methicillin-resistant S. aureus (MRSA, MIC=256 μM). In additional, R2PLx was found to inhibit the proliferation of several tumour cells, especially showing more potent effect on prostate cancer cell, PC-3. The early cell apoptosis was observed in 6 h by Annexin V-FITC/PI staining, as well as the activation of Caspase-3 at 5 μM peptide concentration. R2PLx may therefore be promising for developing new therapeutic approach for cancer treatment. In addition, the artificial deficiency of conserved rana-box loop or net positive charge in C-terminal domain notably reduced the biological activities of the truncated and substituted isoforms, respectively, suggesting for maintaining their biological potency of Ranatuerin family requires both cysteine-bridged segment and cationincity within the loop domain in C-terminus.",
keywords = "anuran skin secretions, peptides, antibacterial properties, anti-cancer, rana-box",
author = "Xiaoling Chen and Luyao Zhang and Chengbang Ma and Yingqi Zhang and Xinping Xi and Lei Wang and Mei Zhou and Burrows, {James F.} and Tianbao Chen",
year = "2018",
month = "12",
doi = "10.1042/BSR20180710",
language = "English",
volume = "38",
journal = "Bioscience Reports",
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TY - JOUR

T1 - A novel antimicrobial peptide, Ranatuerin-2PLx (R2PLx), showing therapeutic potential in inhibiting proliferation of cancer cells

AU - Chen, Xiaoling

AU - Zhang, Luyao

AU - Ma, Chengbang

AU - Zhang, Yingqi

AU - Xi, Xinping

AU - Wang, Lei

AU - Zhou, Mei

AU - Burrows, James F.

AU - Chen, Tianbao

PY - 2018/12

Y1 - 2018/12

N2 - Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, a 28-mer polypeptide, R2PLx, was identified from lyophilised skin secretions. The chemically-synthetic replicates exhibited moderate and broad-spectrum antimicrobial effect against various microorganisms including methicillin-resistant S. aureus (MRSA, MIC=256 μM). In additional, R2PLx was found to inhibit the proliferation of several tumour cells, especially showing more potent effect on prostate cancer cell, PC-3. The early cell apoptosis was observed in 6 h by Annexin V-FITC/PI staining, as well as the activation of Caspase-3 at 5 μM peptide concentration. R2PLx may therefore be promising for developing new therapeutic approach for cancer treatment. In addition, the artificial deficiency of conserved rana-box loop or net positive charge in C-terminal domain notably reduced the biological activities of the truncated and substituted isoforms, respectively, suggesting for maintaining their biological potency of Ranatuerin family requires both cysteine-bridged segment and cationincity within the loop domain in C-terminus.

AB - Antimicrobial peptides are a promising resource for developing novel antibiotic and even anticancer drugs. Here, a 28-mer polypeptide, R2PLx, was identified from lyophilised skin secretions. The chemically-synthetic replicates exhibited moderate and broad-spectrum antimicrobial effect against various microorganisms including methicillin-resistant S. aureus (MRSA, MIC=256 μM). In additional, R2PLx was found to inhibit the proliferation of several tumour cells, especially showing more potent effect on prostate cancer cell, PC-3. The early cell apoptosis was observed in 6 h by Annexin V-FITC/PI staining, as well as the activation of Caspase-3 at 5 μM peptide concentration. R2PLx may therefore be promising for developing new therapeutic approach for cancer treatment. In addition, the artificial deficiency of conserved rana-box loop or net positive charge in C-terminal domain notably reduced the biological activities of the truncated and substituted isoforms, respectively, suggesting for maintaining their biological potency of Ranatuerin family requires both cysteine-bridged segment and cationincity within the loop domain in C-terminus.

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JO - Bioscience Reports

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