A novel experimental approach to investigate radiolysis processes in liquid samples using collimated radiation sources

Chris Polin, Nathan Wardlow, Harold McQuaid, Philip Orr, Balder Villagomez-Bernabe, Catarina Figueira, Grace Alexander, Shajeth Srigengan, Emilie Brun, Manon Gilles, Cécile Sicard-Roselli, Fred J. Currell

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Abstract

Here is detailed a novel and low-cost experimental method for high-throughput automated fluid sample irradiation. The sample is delivered via syringe pump to a nozzle, where it is expressed in the form of a hanging droplet into the path of a beam of ionising radiation. The dose delivery is controlled by an upstream lead shutter, which allows the beam to reach the droplet for a user defined period of time. The droplet is then further expressed after irradiation until it falls into one well of a standard microplate. The entire system is automated and can be operated remotely using software designed in-house, allowing for use in environments deemed unsafe for the user (synchrotron beamlines, for example). Depending on the number of wells in the microplate, several droplets can be irradiated before any human interaction is necessary, and the user may choose up to 10 samples per microplate using an array of identical syringe pumps, the design of which is described here. The nozzles consistently produce droplets of 25.1 ± 0.5 μl.
Original languageEnglish
Article number035106
JournalReview of Scientific Instruments
Volume86
Issue number3
DOIs
Publication statusPublished - Mar 2015

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Radiolysis
radiolysis
radiation sources
syringes
Radiation
nozzles
Liquids
liquids
Syringes
pumps
irradiation
shutters
Nozzles
ionizing radiation
upstream
Irradiation
Pumps
delivery
synchrotrons
computer programs

Cite this

Polin, Chris ; Wardlow, Nathan ; McQuaid, Harold ; Orr, Philip ; Villagomez-Bernabe, Balder ; Figueira, Catarina ; Alexander, Grace ; Srigengan, Shajeth ; Brun, Emilie ; Gilles, Manon ; Sicard-Roselli, Cécile ; Currell, Fred J. / A novel experimental approach to investigate radiolysis processes in liquid samples using collimated radiation sources. In: Review of Scientific Instruments. 2015 ; Vol. 86, No. 3.
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Polin, C, Wardlow, N, McQuaid, H, Orr, P, Villagomez-Bernabe, B, Figueira, C, Alexander, G, Srigengan, S, Brun, E, Gilles, M, Sicard-Roselli, C & Currell, FJ 2015, 'A novel experimental approach to investigate radiolysis processes in liquid samples using collimated radiation sources', Review of Scientific Instruments, vol. 86, no. 3, 035106. https://doi.org/10.1063/1.4914054

A novel experimental approach to investigate radiolysis processes in liquid samples using collimated radiation sources. / Polin, Chris; Wardlow, Nathan; McQuaid, Harold; Orr, Philip; Villagomez-Bernabe, Balder; Figueira, Catarina; Alexander, Grace; Srigengan, Shajeth; Brun, Emilie; Gilles, Manon; Sicard-Roselli, Cécile; Currell, Fred J.

In: Review of Scientific Instruments, Vol. 86, No. 3, 035106, 03.2015.

Research output: Contribution to journalArticle

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AU - Alexander, Grace

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