A novel human pain insensitivity disorder caused by a point mutation in ZFHX2

Abdella M Habib, Ayako Matsuyama, Andrei L Okorokov, Sonia Santana-Varela, Jose T Bras, Anna Maria Aloisi, Edward C Emery, Yury D Bogdanov, Maryne Follenfant, Sam J Gossage, Mathilde Gras, Jack Humphrey, Anna Kolesnikov, Kim Le Cann, Shengnan Li, Michael S Minett, Vanessa Pereira, Clara Ponsolles, Shafaq Sikandar, Jesus M TorresKenji Yamaoka, Jing Zhao, Yuriko Komine, Tetsuo Yamamori, Nikolas Maniatis, Konstantin I Panov, Henry Houlden, Juan D Ramirez, David L H Bennett, Letizia Marsili, Valeria Bachiocco, John N Wood, James J Cox

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Abstract

Chronic pain is a major global public health issue causing a severe impact on both the quality of life for sufferers and the wider economy. Despite the significant clinical burden, little progress has been made in terms of therapeutic development. A unique approach to identifying new human-validated analgesic drug targets is to study rare families with inherited pain insensitivity. Here we have analysed an otherwise normal family where six affected individuals display a pain insensitive phenotype that is characterized by hyposensitivity to noxious heat and painless bone fractures. This autosomal dominant disorder is found in three generations and is not associated with a peripheral neuropathy. A novel point mutation in ZFHX2, encoding a putative transcription factor expressed in small diameter sensory neurons, was identified by whole exome sequencing that segregates with the pain insensitivity. The mutation is predicted to change an evolutionarily highly conserved arginine residue 1913 to a lysine within a homeodomain. Bacterial artificial chromosome (BAC) transgenic mice bearing the orthologous murine p.R1907K mutation, as well as Zfhx2 null mutant mice, have significant deficits in pain sensitivity. Gene expression analyses in dorsal root ganglia from mutant and wild-type mice show altered expression of genes implicated in peripheral pain mechanisms. The ZFHX2 variant and downstream regulated genes associated with a human pain-insensitive phenotype are therefore potential novel targets for the development of new analgesic drugs.awx326media15680039660001.

Original languageEnglish
JournalBrain
Early online date14 Dec 2017
DOIs
Publication statusEarly online date - 14 Dec 2017
Externally publishedYes

Keywords

  • Journal Article

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