A novel method of quantifying root exudation in the presence of soil microflora

A. A. Meharg*, K. Killham

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)


A microcosm is described in which root exudation may be estimated in the presence of microorganisms. Ryegrass seedlings are grown in microcosms in which roots were spatially separated from a microbial inoculant by a Millipore membrane. Seedlings grown in the microcosms were labelled with [14C]-CO2, and the fate of the label within the plant and rhizosphere was determined. Inoculation of the microcosms with Cladosporium resinae increased net fixation of the [14C] label compared to plants grown under sterile conditions. Inoculation also increased root exudation. The use of the microcosm was illustrated and its applications discussed.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalPlant and Soil
Issue number1
Publication statusPublished - May 1991


  • Lolium perenne
  • rhizosphere
  • root exudation

ASJC Scopus subject areas

  • Soil Science
  • Plant Science
  • Agronomy and Crop Science
  • General Earth and Planetary Sciences
  • General Environmental Science


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