A novel subpopulation of monocyte-like cells in the human lung after lipopolysaccharide inhalation

Mairi Brittan, Laura Barr, Andrew Conway Morris, Rodger Duffin, Fiona Rossi, Shonna Johnston, Graham Monro, Niall Anderson, Adriano G. Rossi, Danny F. McAuley, Christopher Haslett, Nik Hirani, Kev Dhaliwal, A. John Simpson

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19 Citations (Scopus)

Abstract

The co-ordinated recruitment of monocyte subpopulations, neutrophils and regulatory T-cells (Tregs) during the early stages of human acute lung inflammation remains poorly understood. We therefore performed a detailed characterisation of these lineages in the blood and lungs in a model of human acute lung inflammation.

Healthy volunteers inhaled lipopolysaccharide (LPS) or saline (n=6 for each group). Blood was collected at 0, 2, 4, 6 and 8 h and bronchoscopy with bronchoalveolar lavage (BAL) performed at 8 h. Multiparameter flow cytometry was used to characterise monocyte subpopulations, neutrophils and Tregs in the blood and lung.

Inhalation of LPS was associated with significant blood and BAL fluid neutrophilia. Blood populations of monocyte subpopulations and Tregs were unaltered by LPS. In contrast, LPS induced an accumulation of a pulmonary monocyte-like cell (PMLC) population, which was further subdivided into “inducible” CD14++CD16- and “resident” CD14++CD16+ subsets. Inducible PMLCs were significantly increased following LPS inhalation (p=0.0046), whereas resident PMLCs were unchanged. In addition, we noted a significant decrease in Tregs in BAL fluid with LPS inhalation (p=0.027).

The early stages of LPS-induced inflammation in humans is characterised by pulmonary accumulation of a novel inducible monocyte-like subpopulation, accompanied by significant changes in both neutrophil and Treg numbers.
Original languageEnglish
Pages (from-to)206-214
Number of pages9
JournalEuropean Respiratory Journal
Volume40
Issue number1
Early online date20 Jan 2012
DOIs
Publication statusPublished - 01 Jul 2012

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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    Brittan, M., Barr, L., Conway Morris, A., Duffin, R., Rossi, F., Johnston, S., Monro, G., Anderson, N., Rossi, A. G., McAuley, D. F., Haslett, C., Hirani, N., Dhaliwal, K., & Simpson, A. J. (2012). A novel subpopulation of monocyte-like cells in the human lung after lipopolysaccharide inhalation. European Respiratory Journal, 40(1), 206-214. https://doi.org/10.1183/09031936.00113811