A PLATE ASSAY FOR THE DETECTION OF ORGANOPHOSPHONATE MINERALIZATION BY ENVIRONMENTAL BACTERIA, AND ITS MODIFICATION AS AN ACTIVITY STAIN FOR IDENTIFICATION OF THE CARBON-PHOSPHORUS BOND-CLEAVAGE ENZYME PHOSPHONOACETATE HYDROLASE

J P  QUINN; John McGrath

A PLATE ASSAY FOR THE DETECTION OF ORGANOPHOSPHONATE MINERALIZATION BY ENVIRONMENTAL BACTERIA, AND ITS MODIFICATION AS AN ACTIVITY STAIN FOR IDENTIFICATION OF THE CARBON-PHOSPHORUS BOND-CLEAVAGE ENZYME PHOSPHONOACETATE HYDROLASE

J P QUINN, John McGrath

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A replica plate screening technique, based on the acid molybdate assay for detection of phosphate has been developed to permit the detection of microorganisms capable of mineralizing organophosphonates. The method was further adapted as the basis of an activity stain for the detection of the carbon - phosphorus bond cleavage enzyme phosphonoacetate hydrolase in PAGE gels.

Original language	English
Pages (from-to)	497-502
Number of pages	6
Journal	Biotechnology techniques
Volume	9
Issue number	7
Publication status	Published - Jul 1995

Bibliographical note

(Corresponding Author: Devised and carried out experiments).

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@article{6f91284528c14d1a9c2ade4558a591e6,

title = "A PLATE ASSAY FOR THE DETECTION OF ORGANOPHOSPHONATE MINERALIZATION BY ENVIRONMENTAL BACTERIA, AND ITS MODIFICATION AS AN ACTIVITY STAIN FOR IDENTIFICATION OF THE CARBON-PHOSPHORUS BOND-CLEAVAGE ENZYME PHOSPHONOACETATE HYDROLASE",

abstract = "A replica plate screening technique, based on the acid molybdate assay for detection of phosphate has been developed to permit the detection of microorganisms capable of mineralizing organophosphonates. The method was further adapted as the basis of an activity stain for the detection of the carbon - phosphorus bond cleavage enzyme phosphonoacetate hydrolase in PAGE gels.",

author = "QUINN, {J P} and John McGrath",

note = "(Corresponding Author: Devised and carried out experiments). ",

year = "1995",

month = jul,

language = "English",

volume = "9",

pages = "497--502",

journal = "Biotechnology techniques",

issn = "0951-208X",

publisher = "Kluwer Academic Publishers",

number = "7",

}

A PLATE ASSAY FOR THE DETECTION OF ORGANOPHOSPHONATE MINERALIZATION BY ENVIRONMENTAL BACTERIA, AND ITS MODIFICATION AS AN ACTIVITY STAIN FOR IDENTIFICATION OF THE CARBON-PHOSPHORUS BOND-CLEAVAGE ENZYME PHOSPHONOACETATE HYDROLASE. / QUINN, J P ; McGrath, John.
In: Biotechnology techniques, Vol. 9, No. 7, 07.1995, p. 497-502.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A PLATE ASSAY FOR THE DETECTION OF ORGANOPHOSPHONATE MINERALIZATION BY ENVIRONMENTAL BACTERIA, AND ITS MODIFICATION AS AN ACTIVITY STAIN FOR IDENTIFICATION OF THE CARBON-PHOSPHORUS BOND-CLEAVAGE ENZYME PHOSPHONOACETATE HYDROLASE

AU - QUINN, J P

AU - McGrath, John

N1 - (Corresponding Author: Devised and carried out experiments).

PY - 1995/7

Y1 - 1995/7

N2 - A replica plate screening technique, based on the acid molybdate assay for detection of phosphate has been developed to permit the detection of microorganisms capable of mineralizing organophosphonates. The method was further adapted as the basis of an activity stain for the detection of the carbon - phosphorus bond cleavage enzyme phosphonoacetate hydrolase in PAGE gels.

AB - A replica plate screening technique, based on the acid molybdate assay for detection of phosphate has been developed to permit the detection of microorganisms capable of mineralizing organophosphonates. The method was further adapted as the basis of an activity stain for the detection of the carbon - phosphorus bond cleavage enzyme phosphonoacetate hydrolase in PAGE gels.

M3 - Article

SN - 0951-208X

VL - 9

SP - 497

EP - 502

JO - Biotechnology techniques

JF - Biotechnology techniques

IS - 7

ER -