A potentiometric biosensor for rapid on-site disease diagnostics

Alexey Tarasov, Darren W. Gray, Meng-Yen Tsai, Niall Shields, Armele Montrose, Niamh Creedon, Pierre Lovera, Alan O'Riordan, Mark H. Mooney, Eric M. Vogel

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49 Citations (Scopus)

Abstract

Quantitative point-of-care (POC) devices are the next generation for serological disease diagnosis. Whilst pathogen serology is typically performed by centralized laboratories using Enzyme-Linked ImmunoSorbent Assay (ELISA), faster on-site diagnosis would infer improved disease management and treatment decisions. Using the model pathogen Bovine Herpes Virus-1 (BHV-1) this study employs an extended-gate field-effect transistor (FET) for direct potentiometric serological diagnosis. BHV-1 is a major viral pathogen of Bovine Respiratory Disease (BRD), the leading cause of economic loss ($2 billion annually in the US only) to the cattle and dairy industry. To demonstrate the sensor capabilities as a diagnostic tool, BHV-1 viral protein gE was expressed and immobilized on the sensor surface to serve as a capture antigen for a BHV-1-specific antibody (anti-gE), produced in cattle in response to viral infection. The gE-coated immunosensor was shown to be highly sensitive and selective to anti-gE present in commercially available anti-BHV-1 antiserum and in real serum samples from cattle with results being in excellent agreement with Surface Plasmon Resonance (SPR) and ELISA. The FET sensor is significantly faster than ELISA (<10 min), a crucial factor for successful disease intervention. This sensor technology is versatile, amenable to multiplexing, easily integrated to POC devices, and has the potential to impact a wide range of human and animal diseases.
Original languageEnglish
Pages (from-to)669-678
Number of pages10
JournalBiosensors and Bioelectronics
Volume79
Early online date24 Dec 2015
DOIs
Publication statusPublished - 15 May 2016

Keywords

  • Field-effect transistors
  • Potentiometric biosensors
  • Immunosensors
  • Serological diagnostics
  • Bovine Respiratory Disease
  • Bovine Herpes Virus-1

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  • Cite this

    Tarasov, A., Gray, D. W., Tsai, M-Y., Shields, N., Montrose, A., Creedon, N., Lovera, P., O'Riordan, A., Mooney, M. H., & Vogel, E. M. (2016). A potentiometric biosensor for rapid on-site disease diagnostics. Biosensors and Bioelectronics, 79, 669-678. https://doi.org/10.1016/j.bios.2015.12.086