A practical miniature long-term bone marrow culture system for investigating early myelodysplasia

I A Roberts; Mary Frances McMullin

doi:10.1016/0145-2126(92)90151-V

A practical miniature long-term bone marrow culture system for investigating early myelodysplasia

I A Roberts, Mary Frances McMullin

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A method was devised to grow haemopoietic cells in long-term bone marrow culture (LTBMC) which requires only 1 x 10(6) cells/culture. Such miniature cultures were used to study growth patterns of marrow from patients with myelodysplastic syndromes (MDS). Consistent differences in LTBMC cellularity and cellular composition were noted between MDS and normal marrow. These differences were accentuated by rGM-CSF. The criteria which distinguished between and MDS marrows were: cell count at weeks 1 and 4, % neutrophils and % blasts. In 10 patients with unexplained macrocytosis or pancytopenia miniature LTBMC results clearly segregated into either 'normal' or 'MDS' growth patterns. Miniature LTBMC with rGM-CSF may therefore be a useful diagnostic test for early MDS.

Original language	English
Pages (from-to)	737-41
Number of pages	5
Journal	Leukemia research
Volume	16
Issue number	8
DOIs	https://doi.org/10.1016/0145-2126(92)90151-V
Publication status	Published - Aug 1992

Keywords

Adult
Analysis of Variance
Bone Marrow
Cells, Cultured
Colony-Forming Units Assay
Female
Granulocyte-Macrophage Colony-Stimulating Factor
Hematopoietic Stem Cells
Humans
Male
Middle Aged
Myelodysplastic Syndromes
Predictive Value of Tests
Recombinant Proteins
Time Factors

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10.1016/0145-2126(92)90151-V

Cite this

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title = "A practical miniature long-term bone marrow culture system for investigating early myelodysplasia",

abstract = "A method was devised to grow haemopoietic cells in long-term bone marrow culture (LTBMC) which requires only 1 x 10(6) cells/culture. Such miniature cultures were used to study growth patterns of marrow from patients with myelodysplastic syndromes (MDS). Consistent differences in LTBMC cellularity and cellular composition were noted between MDS and normal marrow. These differences were accentuated by rGM-CSF. The criteria which distinguished between and MDS marrows were: cell count at weeks 1 and 4, % neutrophils and % blasts. In 10 patients with unexplained macrocytosis or pancytopenia miniature LTBMC results clearly segregated into either 'normal' or 'MDS' growth patterns. Miniature LTBMC with rGM-CSF may therefore be a useful diagnostic test for early MDS.",

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author = "Roberts, {I A} and McMullin, {Mary Frances}",

year = "1992",

month = aug,

doi = "10.1016/0145-2126(92)90151-V",

language = "English",

volume = "16",

pages = "737--41",

journal = "Leukemia research",

issn = "0145-2126",

publisher = "Elsevier Ltd",

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}

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T1 - A practical miniature long-term bone marrow culture system for investigating early myelodysplasia

AU - Roberts, I A

AU - McMullin, Mary Frances

PY - 1992/8

Y1 - 1992/8

N2 - A method was devised to grow haemopoietic cells in long-term bone marrow culture (LTBMC) which requires only 1 x 10(6) cells/culture. Such miniature cultures were used to study growth patterns of marrow from patients with myelodysplastic syndromes (MDS). Consistent differences in LTBMC cellularity and cellular composition were noted between MDS and normal marrow. These differences were accentuated by rGM-CSF. The criteria which distinguished between and MDS marrows were: cell count at weeks 1 and 4, % neutrophils and % blasts. In 10 patients with unexplained macrocytosis or pancytopenia miniature LTBMC results clearly segregated into either 'normal' or 'MDS' growth patterns. Miniature LTBMC with rGM-CSF may therefore be a useful diagnostic test for early MDS.

AB - A method was devised to grow haemopoietic cells in long-term bone marrow culture (LTBMC) which requires only 1 x 10(6) cells/culture. Such miniature cultures were used to study growth patterns of marrow from patients with myelodysplastic syndromes (MDS). Consistent differences in LTBMC cellularity and cellular composition were noted between MDS and normal marrow. These differences were accentuated by rGM-CSF. The criteria which distinguished between and MDS marrows were: cell count at weeks 1 and 4, % neutrophils and % blasts. In 10 patients with unexplained macrocytosis or pancytopenia miniature LTBMC results clearly segregated into either 'normal' or 'MDS' growth patterns. Miniature LTBMC with rGM-CSF may therefore be a useful diagnostic test for early MDS.

KW - Adult

KW - Analysis of Variance

KW - Bone Marrow

KW - Cells, Cultured

KW - Colony-Forming Units Assay

KW - Female

KW - Granulocyte-Macrophage Colony-Stimulating Factor

KW - Hematopoietic Stem Cells

KW - Humans

KW - Male

KW - Middle Aged

KW - Myelodysplastic Syndromes

KW - Predictive Value of Tests

KW - Recombinant Proteins

KW - Time Factors

U2 - 10.1016/0145-2126(92)90151-V

DO - 10.1016/0145-2126(92)90151-V

M3 - Article

C2 - 1528061

SN - 0145-2126

VL - 16

SP - 737

EP - 741

JO - Leukemia research

JF - Leukemia research

IS - 8

ER -

A practical miniature long-term bone marrow culture system for investigating early myelodysplasia

Abstract

Keywords

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