A primer on the current state of microarray technologies

Alexander J. Trachtenberg, Jae Hyung Robert, Azza E. Abdalla, Andrew Fraser, Steven Y. He, Jessica N. Lacy, Chiara Rivas-Morello, Allison Truong, Gary Hardiman, Lucila Ohno-Machado, Fang Liu, Eivind Hovig, Winston Patrick Kuo*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Citations (Scopus)

Abstract

DNA microarray technology has been used for genome-wide gene expression studies that incorporate molecular genetics and computer science analyses on massive levels. The availability of microarrays permit the simultaneous analysis of tens of thousands of genes for the purposes of gene discovery, disease diagnosis, improved drug development, and therapeutics tailored to specific disease processes. In this chapter, we provide an overview on the current state of common microarray technologies and platforms. Since many genes contribute to normal functioning, research efforts are moving from the search for a disease-specific gene to the understanding of the biochemical and molecular functioning of a variety of genes whose disrupted interaction in complicated networks can lead to a disease state. The field of microarrays has evolved over the past decade and is now standardized with a high level of quality control, while providing a relatively inexpensive and reliable alternative to studying various aspects of gene expression.

Original languageEnglish
Title of host publicationNext Generation Microarray Bioinformatics
Subtitle of host publicationMethods and Protocols
EditorsJunbai Wang, Tianhai Tian, Aik Choon Tan
Pages3-17
Number of pages15
DOIs
Publication statusPublished - 02 Jan 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume802
ISSN (Print)1064-3745

Keywords

  • Cross platform
  • Gene expression
  • High throughput
  • Microarrays
  • One dye
  • QRT-PCR
  • Two dye

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Trachtenberg, A. J., Robert, J. H., Abdalla, A. E., Fraser, A., He, S. Y., Lacy, J. N., Rivas-Morello, C., Truong, A., Hardiman, G., Ohno-Machado, L., Liu, F., Hovig, E., & Kuo, W. P. (2012). A primer on the current state of microarray technologies. In J. Wang, T. Tian, & A. C. Tan (Eds.), Next Generation Microarray Bioinformatics: Methods and Protocols (pp. 3-17). (Methods in Molecular Biology; Vol. 802). https://doi.org/10.1007/978-1-61779-400-1_1