A putative gene cluster for aminoarabinose biosynthesis is essential for Burkholderia cenocepacia viability

Ximena P Ortega, Silvia T Cardona, Alan R Brown, Slade A Loutet, Ronald S Flannagan, Dominic J Campopiano, John R W Govan, Miguel A Valvano

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Abstract

Using a conditional mutagenesis strategy we demonstrate here that a gene cluster encoding putative aminoarabinose (Ara4N) biosynthesis enzymes is essential for the viability of Burkholderia cenocepacia. Loss of viability is associated with dramatic changes in bacterial cell morphology and ultrastructure, increased permeability to propidium iodide, and sensitivity to sodium dodecyl sulfate, suggesting a general cell envelope defect caused by the lack of Ara4N.
Original languageEnglish
Pages (from-to)3639-44
Number of pages6
JournalJournal of Bacteriology
Volume189
Issue number9
DOIs
Publication statusPublished - May 2007

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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    Ortega, X. P., Cardona, S. T., Brown, A. R., Loutet, S. A., Flannagan, R. S., Campopiano, D. J., Govan, J. R. W., & Valvano, M. A. (2007). A putative gene cluster for aminoarabinose biosynthesis is essential for Burkholderia cenocepacia viability. Journal of Bacteriology, 189(9), 3639-44. https://doi.org/10.1128/JB.00153-07