Abstract
1. Environmental (e)DNA assays are becoming increasingly used to detect rare or invasive aquatic species.
2. The Critically Endangered freshwater pearl mussel Margaritifera margaritifera is undergoing range-wide reduction in population numbers and distribution.
3. An eDNA assay to detect the presence of M. margaritifera was developed, based on the mitochondrial cytochrome oxidase I gene, utilizing species-specific primers, a minor groove binding (MGB) probe and quantitative (q)PCR approaches.
4. The results from this pilot study demonstrated high sensitivity both in laboratory and field trials, and provide a valuable non-invasive tool for detecting M. margaritifera.
2. The Critically Endangered freshwater pearl mussel Margaritifera margaritifera is undergoing range-wide reduction in population numbers and distribution.
3. An eDNA assay to detect the presence of M. margaritifera was developed, based on the mitochondrial cytochrome oxidase I gene, utilizing species-specific primers, a minor groove binding (MGB) probe and quantitative (q)PCR approaches.
4. The results from this pilot study demonstrated high sensitivity both in laboratory and field trials, and provide a valuable non-invasive tool for detecting M. margaritifera.
Original language | English |
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Journal | Aquatic Conservation Marine and Freshwater Ecosystems |
Early online date | 12 Jul 2017 |
DOIs | |
Publication status | Early online date - 12 Jul 2017 |
Keywords
- Conservation
- Detection
- Environmental DNA
- Monitoring
- Non-invasive
- Quantitative PCR
ASJC Scopus subject areas
- Ecology
- Aquatic Science
- Nature and Landscape Conservation