A qPCR MGB probe based eDNA assay for European freshwater pearl mussel (Margaritifera margaritifera L.)

Jeanette E L Carlsson, Damian Egan, Patrick C. Collins, Edward D. Farrell, Fran Igoe, Jens Carlsson*

*Corresponding author for this work

Research output: Contribution to journalArticle

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Abstract

1. Environmental (e)DNA assays are becoming increasingly used to detect rare or invasive aquatic species.
2. The Critically Endangered freshwater pearl mussel Margaritifera margaritifera is undergoing range-wide reduction in population numbers and distribution.
3. An eDNA assay to detect the presence of M. margaritifera was developed, based on the mitochondrial cytochrome oxidase I gene, utilizing species-specific primers, a minor groove binding (MGB) probe and quantitative (q)PCR approaches.
4. The results from this pilot study demonstrated high sensitivity both in laboratory and field trials, and provide a valuable non-invasive tool for detecting M. margaritifera.
Original languageEnglish
JournalAquatic Conservation Marine and Freshwater Ecosystems
Early online date12 Jul 2017
DOIs
Publication statusEarly online date - 12 Jul 2017

Keywords

  • Conservation
  • Detection
  • Environmental DNA
  • Monitoring
  • Non-invasive
  • Quantitative PCR

ASJC Scopus subject areas

  • Ecology
  • Aquatic Science
  • Nature and Landscape Conservation

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