A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs

Guerrino Macori, Siobhán C. McCarthy, Catherine M. Burgess*, Séamus Fanning, Geraldine Duffy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values to the initial concentration of pathogen in the sheep sample.

Original languageEnglish
Article number105703
JournalJournal of microbiological methods
Volume165
Early online date10 Sep 2019
DOIs
Publication statusPublished - Oct 2019
Externally publishedYes

Bibliographical note

Funding Information:
This project was funded by the Food Institutional Research Measure , administered by the Irish Department of Agriculture, Food and the Marine (project n. 15/F/629 ).

Funding Information:
This project was funded by the Food Institutional Research Measure, administered by the Irish Department of Agriculture, Food and the Marine (project n. 15/F/629).

Publisher Copyright:
© 2019 Elsevier B.V.

Keywords

  • Escherichia coli O157
  • Escherichia coli O26
  • Quantitative real-time PCR
  • Sheep

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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