Abstract
A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values to the initial concentration of pathogen in the sheep sample.
Original language | English |
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Article number | 105703 |
Journal | Journal of microbiological methods |
Volume | 165 |
Early online date | 10 Sept 2019 |
DOIs | |
Publication status | Published - Oct 2019 |
Externally published | Yes |
Bibliographical note
Funding Information:This project was funded by the Food Institutional Research Measure , administered by the Irish Department of Agriculture, Food and the Marine (project n. 15/F/629 ).
Funding Information:
This project was funded by the Food Institutional Research Measure, administered by the Irish Department of Agriculture, Food and the Marine (project n. 15/F/629).
Publisher Copyright:
© 2019 Elsevier B.V.
Keywords
- Escherichia coli O157
- Escherichia coli O26
- Quantitative real-time PCR
- Sheep
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Microbiology (medical)