A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs

Guerrino Macori; Siobhán C. McCarthy; Catherine M. Burgess; Séamus Fanning; Geraldine Duffy

doi:10.1016/j.mimet.2019.105703

A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs

Guerrino Macori
, Siobhán C. McCarthy
, Catherine M. Burgess^*
, Séamus Fanning
, Geraldine Duffy

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values to the initial concentration of pathogen in the sheep sample.

Original language	English
Article number	105703
Journal	Journal of microbiological methods
Volume	165
Early online date	10 Sept 2019
DOIs	https://doi.org/10.1016/j.mimet.2019.105703
Publication status	Published - Oct 2019
Externally published	Yes

Bibliographical note

Funding Information:
This project was funded by the Food Institutional Research Measure , administered by the Irish Department of Agriculture, Food and the Marine (project n. 15/F/629 ).

Funding Information:
This project was funded by the Food Institutional Research Measure, administered by the Irish Department of Agriculture, Food and the Marine (project n. 15/F/629).

Publisher Copyright:
© 2019 Elsevier B.V.

Keywords

Escherichia coli O157
Escherichia coli O26
Quantitative real-time PCR
Sheep

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2019.105703Licence: Unspecified

Cite this

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title = "A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs",

abstract = "A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values to the initial concentration of pathogen in the sheep sample.",

keywords = "Escherichia coli O157, Escherichia coli O26, Quantitative real-time PCR, Sheep",

author = "Guerrino Macori and McCarthy, \{Siobh{\'a}n C.\} and Burgess, \{Catherine M.\} and S{\'e}amus Fanning and Geraldine Duffy",

note = "Funding Information: This project was funded by the Food Institutional Research Measure , administered by the Irish Department of Agriculture, Food and the Marine (project n. 15/F/629 ). Funding Information: This project was funded by the Food Institutional Research Measure, administered by the Irish Department of Agriculture, Food and the Marine (project n. 15/F/629). Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

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AU - Macori, Guerrino

AU - McCarthy, Siobhán C.

AU - Burgess, Catherine M.

AU - Fanning, Séamus

AU - Duffy, Geraldine

N1 - Funding Information: This project was funded by the Food Institutional Research Measure , administered by the Irish Department of Agriculture, Food and the Marine (project n. 15/F/629 ). Funding Information: This project was funded by the Food Institutional Research Measure, administered by the Irish Department of Agriculture, Food and the Marine (project n. 15/F/629). Publisher Copyright: © 2019 Elsevier B.V.

PY - 2019/10

Y1 - 2019/10

N2 - A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values to the initial concentration of pathogen in the sheep sample.

AB - A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values to the initial concentration of pathogen in the sheep sample.

KW - Escherichia coli O157

KW - Escherichia coli O26

KW - Quantitative real-time PCR

KW - Sheep

U2 - 10.1016/j.mimet.2019.105703

DO - 10.1016/j.mimet.2019.105703

M3 - Article

C2 - 31454506

AN - SCOPUS:85071937827

SN - 0167-7012

VL - 165

JO - Journal of microbiological methods

JF - Journal of microbiological methods

M1 - 105703

ER -

A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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Cite this