Abstract
Specific antibodies are essential components of immunoassay, which can be applied for the detection of pathogens. However, producing an antibody specific to live bacterial pathogens by the classical method of immunizing animals with live pathogens can be impractical. Phage display technology is an effective alternative method to obtain antibodies with the desired specificity against selected antigenic molecules. In this study, we demonstrated the power of a microarray-based technique for obtaining specific phage-derived antibody fragments against , an important foodborne pathogen. The selected phage-displayed antibody fragments were subsequently employed to develop a lateral flow test strip assay for the detection of live . The test strips showed specificity to Enteritidis without cross-reactivity to eight serovars of or other bacteria strains. The test strip assay requires 15 min, whereas the conventional biochemical and serological confirmation test requires at least 24 h. The microarray screening technique for specific phage-based binders and the test strip method can be further applied to other foodborne pathogens.
| Original language | English |
|---|---|
| Article number | 1008817 |
| Journal | Frontiers in Microbiology |
| Volume | 13 |
| DOIs | |
| Publication status | Published - 29 Sept 2022 |
Keywords
- phage-derived antibody fragment
- Salmonella Enteritidis
- lateral flow assay
- foodborne pathogen
- colorimetric assay