A rapid HPLC protocol for detection of polyols and trehalose

John E. Hallsworth*, Naresh Magan

*Corresponding author for this work

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A procedure was developed to extract polyols and trehalose (protectants against stress) from fungal conidia. Conidia were sonicated (120 s) and immersed in a boiling water bath (5.5 min) to optimize extraction of polyols and trehalose, respectively. A rapid method was developed to separate and detect low-molecular-weight polyols and trehalose using high-performance liquid chromatography (HPLC). An ion exchange column designed for standard carbohydrate analysis was used in preference to one designed for sugar alcohol separation. This resulted in rapid elution (less than 5 min), without sacrificing peak resolution. The use of a pulsed electrochemical detector (gold electrode) resulted in limits of reliable quantification as low as 1.6 μg ml-1 for polyols and 2.8 μg ml-1 for trehalose. This is very sensitive and rapid method by which these protectants can be analysed. It avoids polyol derivatization that characterizes analysis by gas chromatography and the long run times (up to 45 min) that typify HPLC analysis using sugar alcohol columns.
Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalJournal of Microbiological Methods
Volume29
Issue number1
DOIs
Publication statusPublished - 01 Apr 1997

Keywords

  • Compatible solutes
  • Conidia
  • Fungi
  • High-performance liquid chromatography
  • Polyols
  • Trehalose

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

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