A specificity-enhanced time-resolved fluoroimmunoassay for zeranol employing the dry reagent all-in-one-well principle

M. Tuomola, Kevin M Cooper, S. Lahdenpera, Robert Baxter, Christopher Elliott, Diarmuid Kennedy, T. Lovgren

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A simple dry chemistry time-resolved fluorescence immunoassay (TR-FIA) method was developed for the measurement of zeranol in bovine urine samples. The samples were purified by immunoaffinity chromatography and a specificity-enhanced zeranol antibody was employed in the immunoassay. This resulted in a highly selective method, which had only negligible reactivity with Fusarium spp, toxins. The all-in-one-well dry chemistry concept made the assay very simple to use because all the assay-specific reagents were already present in the reaction wells in dry form. Only the addition of diluted sample extract was required to perform the competitive one-step TR-FIA and the results were available in less than 1 h. The analytical limit of detection (mean + 3s) for the immunoassay was 0.16 ng ml(-1) (n=12) and the functional limit of detection for the whole method, estimated by the analysis of zeranol-free samples, was 1.3 ng ml(-1) (n=20). The recovery of zeranol at the level of 2 ng ml(-1) was 99% (n=18) and the within-assay variation ranged between 4.5 and 9.0%.
Original languageEnglish
Pages (from-to)83-86
Number of pages4
JournalAnalyst
Volume127
Issue number1
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'A specificity-enhanced time-resolved fluoroimmunoassay for zeranol employing the dry reagent all-in-one-well principle'. Together they form a unique fingerprint.

  • Cite this