A study of DNA fragmentation patterns in cells irradiated with charged particles: evidence for non-random distributions

Kevin Prise, H.C. Newman, M. Folkard, B.D. Michael

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Many studies have shown that the effectiveness of radiations of varying LET is similar when yields of dsb have been measured, despite large differences in biological response. Recent evidence has suggested however, that current techniques underestimate the yields of dsb. By monitoring the fragmentation of DNA over a wide range of fragment sizes ( 6 Mbp) by pulsed field electrophoresis, RBE values greater than 1.0 for radiations of around 100 keV/mm have been determined. The data provide evidence for the production of correlated breaks produced within cells as particle tracks traverse the nucleus. The highly ordered structure of DNA within mammalian cells may lead to clustering of breaks over distances related to the repeating unit structures of the chromatin. As well as these regionally damaged sites, a major contributor to radiation effectiveness will be the localised clustering of damage in the 1 - 20 bp region. A major effort is required to elucidate the relative importance of these levels of clustering and their importance in biological response.
Original languageEnglish
Pages (from-to)20-23
Number of pages4
JournalPhysica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)
Volume14
Issue numberSUPPL. 1
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Biophysics
  • Physics and Astronomy(all)

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