A T-type channel-calmodulin complex triggers αCaMKII activation

Ileana Micu, Hadhimulya Asmara, Arsalan P. Rizwan,, Giriraj Sahu, Brett A. Simms,, Fang-Xiong Zhang, Jordan D. T. Engbers, Peter K. Stys, Gerald W. Zamponi, Ray W. Turner

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Calmodulin (CaM) is an important signaling molecule that regulates a vast array of cellular functions by activating second messengers involved in cell function and plasticity. Low voltage-activated calcium channels of the Cav3 family have the important role of mediating low threshold calcium influx, but were not believed to interact with CaM. We find a constitutive association between CaM and the Cav3.1 channel at rest that is lost through an activity-dependent and Cav3.1 calcium-dependent CaM dissociation. Moreover, Cav3 calcium influx is sufficient to activate αCaMKII in the cytoplasm in a manner that depends on an intact Cav3.1 C-terminus needed to support the CaM interaction. Our findings thus establish that T-type channel calcium influx invokes a novel dynamic interaction between CaM and Cav3.1 channels to trigger a signaling cascade that leads to αCaMKII activation.
    LanguageEnglish
    Article number37
    Pages1-15
    JournalMolecular Brain
    Volume10
    Issue number1
    DOIs
    Publication statusPublished - 11 Aug 2017

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    Calmodulin
    Calcium
    T-Type Calcium Channels
    Second Messenger Systems
    Calcium Channels
    Cytoplasm

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    Micu, I., Asmara, H., Rizwan, A. P., Sahu, G., Simms, B. A., Zhang, F-X., ... Turner, R. W. (2017). A T-type channel-calmodulin complex triggers αCaMKII activation. Molecular Brain, 10(1), 1-15. [37]. https://doi.org/10.1186/s13041-017-0317-8
    Micu, Ileana ; Asmara, Hadhimulya ; Rizwan, Arsalan P. ; Sahu, Giriraj ; Simms, Brett A. ; Zhang, Fang-Xiong ; Engbers, Jordan D. T. ; Stys, Peter K. ; Zamponi, Gerald W. ; Turner, Ray W. / A T-type channel-calmodulin complex triggers αCaMKII activation. In: Molecular Brain. 2017 ; Vol. 10, No. 1. pp. 1-15.
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    abstract = "Calmodulin (CaM) is an important signaling molecule that regulates a vast array of cellular functions by activating second messengers involved in cell function and plasticity. Low voltage-activated calcium channels of the Cav3 family have the important role of mediating low threshold calcium influx, but were not believed to interact with CaM. We find a constitutive association between CaM and the Cav3.1 channel at rest that is lost through an activity-dependent and Cav3.1 calcium-dependent CaM dissociation. Moreover, Cav3 calcium influx is sufficient to activate αCaMKII in the cytoplasm in a manner that depends on an intact Cav3.1 C-terminus needed to support the CaM interaction. Our findings thus establish that T-type channel calcium influx invokes a novel dynamic interaction between CaM and Cav3.1 channels to trigger a signaling cascade that leads to αCaMKII activation.",
    author = "Ileana Micu and Hadhimulya Asmara and Rizwan,, {Arsalan P.} and Giriraj Sahu and Simms,, {Brett A.} and Fang-Xiong Zhang and Engbers, {Jordan D. T.} and Stys, {Peter K.} and Zamponi, {Gerald W.} and Turner, {Ray W.}",
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    Micu, I, Asmara, H, Rizwan, AP, Sahu, G, Simms, BA, Zhang, F-X, Engbers, JDT, Stys, PK, Zamponi, GW & Turner, RW 2017, 'A T-type channel-calmodulin complex triggers αCaMKII activation', Molecular Brain, vol. 10, no. 1, 37, pp. 1-15. https://doi.org/10.1186/s13041-017-0317-8

    A T-type channel-calmodulin complex triggers αCaMKII activation. / Micu, Ileana; Asmara, Hadhimulya ; Rizwan, Arsalan P. ; Sahu, Giriraj ; Simms, Brett A.; Zhang, Fang-Xiong ; Engbers, Jordan D. T. ; Stys, Peter K. ; Zamponi, Gerald W. ; Turner, Ray W.

    In: Molecular Brain, Vol. 10, No. 1, 37, 11.08.2017, p. 1-15.

    Research output: Contribution to journalArticle

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    AU - Micu, Ileana

    AU - Asmara, Hadhimulya

    AU - Rizwan,, Arsalan P.

    AU - Sahu, Giriraj

    AU - Simms,, Brett A.

    AU - Zhang, Fang-Xiong

    AU - Engbers, Jordan D. T.

    AU - Stys, Peter K.

    AU - Zamponi, Gerald W.

    AU - Turner, Ray W.

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    AB - Calmodulin (CaM) is an important signaling molecule that regulates a vast array of cellular functions by activating second messengers involved in cell function and plasticity. Low voltage-activated calcium channels of the Cav3 family have the important role of mediating low threshold calcium influx, but were not believed to interact with CaM. We find a constitutive association between CaM and the Cav3.1 channel at rest that is lost through an activity-dependent and Cav3.1 calcium-dependent CaM dissociation. Moreover, Cav3 calcium influx is sufficient to activate αCaMKII in the cytoplasm in a manner that depends on an intact Cav3.1 C-terminus needed to support the CaM interaction. Our findings thus establish that T-type channel calcium influx invokes a novel dynamic interaction between CaM and Cav3.1 channels to trigger a signaling cascade that leads to αCaMKII activation.

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    Micu I, Asmara H, Rizwan, AP, Sahu G, Simms, BA, Zhang F-X et al. A T-type channel-calmodulin complex triggers αCaMKII activation. Molecular Brain. 2017 Aug 11;10(1):1-15. 37. https://doi.org/10.1186/s13041-017-0317-8