A time-resolved immunoassay to measure serum antibodies to the rotavirus VP6 capsid protein

Owen Kavanagh; Xi-Lei Zeng; Sasirekha Ramani; Indrani Mukhopadhya; Sue E Crawford; Gagandeep Kang; Mary K Estes

doi:10.1016/j.jviromet.2012.11.003

A time-resolved immunoassay to measure serum antibodies to the rotavirus VP6 capsid protein

Owen Kavanagh, Xi-Lei Zeng, Sasirekha Ramani, Indrani Mukhopadhya, Sue E Crawford, Gagandeep Kang, Mary K Estes

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The rotavirus (RV) inner capsid protein VP6 is widely used to evaluate immune response during natural infection and in vaccine studies. Recombinant VP6 from the most prevalent circulating rotavirus strains in each subgroup (SG) identified in a birth cohort of children in southern India [SGII (G1P[8]) and SGI (G10P[11])] were produced. The purified proteins were used to measure VP6-specific antibodies in a Dissociation-Enhanced Lanthanide Fluorometric Immunoassay (DELFIA). The ability of the assay to detect a =2 fold rise in IgG level in a panel of serum samples from a longitudinal study was compared to a gold standard virus-capture ELISA. A strong association was observed between the assays (p

Original language	English
Pages (from-to)	228-31
Journal	Journal of Virological Methods
Volume	189
Issue number	1
Early online date	23 Nov 2012
DOIs	https://doi.org/10.1016/j.jviromet.2012.11.003
Publication status	Published - Apr 2013

Bibliographical note

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.jviromet.2012.11.003Licence: Unspecified

Cite this

@article{d310875408b64b69a779465299cd02c0,

title = "A time-resolved immunoassay to measure serum antibodies to the rotavirus VP6 capsid protein",

abstract = "The rotavirus (RV) inner capsid protein VP6 is widely used to evaluate immune response during natural infection and in vaccine studies. Recombinant VP6 from the most prevalent circulating rotavirus strains in each subgroup (SG) identified in a birth cohort of children in southern India [SGII (G1P[8]) and SGI (G10P[11])] were produced. The purified proteins were used to measure VP6-specific antibodies in a Dissociation-Enhanced Lanthanide Fluorometric Immunoassay (DELFIA). The ability of the assay to detect a =2 fold rise in IgG level in a panel of serum samples from a longitudinal study was compared to a gold standard virus-capture ELISA. A strong association was observed between the assays (p",

author = "Owen Kavanagh and Xi-Lei Zeng and Sasirekha Ramani and Indrani Mukhopadhya and Crawford, {Sue E} and Gagandeep Kang and Estes, {Mary K}",

year = "2013",

month = apr,

doi = "10.1016/j.jviromet.2012.11.003",

language = "English",

volume = "189",

pages = "228--31",

journal = "Journal of Virological Methods",

issn = "1879-0984",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - A time-resolved immunoassay to measure serum antibodies to the rotavirus VP6 capsid protein

AU - Kavanagh, Owen

AU - Zeng, Xi-Lei

AU - Ramani, Sasirekha

AU - Mukhopadhya, Indrani

AU - Crawford, Sue E

AU - Kang, Gagandeep

AU - Estes, Mary K

PY - 2013/4

Y1 - 2013/4

N2 - The rotavirus (RV) inner capsid protein VP6 is widely used to evaluate immune response during natural infection and in vaccine studies. Recombinant VP6 from the most prevalent circulating rotavirus strains in each subgroup (SG) identified in a birth cohort of children in southern India [SGII (G1P[8]) and SGI (G10P[11])] were produced. The purified proteins were used to measure VP6-specific antibodies in a Dissociation-Enhanced Lanthanide Fluorometric Immunoassay (DELFIA). The ability of the assay to detect a =2 fold rise in IgG level in a panel of serum samples from a longitudinal study was compared to a gold standard virus-capture ELISA. A strong association was observed between the assays (p

AB - The rotavirus (RV) inner capsid protein VP6 is widely used to evaluate immune response during natural infection and in vaccine studies. Recombinant VP6 from the most prevalent circulating rotavirus strains in each subgroup (SG) identified in a birth cohort of children in southern India [SGII (G1P[8]) and SGI (G10P[11])] were produced. The purified proteins were used to measure VP6-specific antibodies in a Dissociation-Enhanced Lanthanide Fluorometric Immunoassay (DELFIA). The ability of the assay to detect a =2 fold rise in IgG level in a panel of serum samples from a longitudinal study was compared to a gold standard virus-capture ELISA. A strong association was observed between the assays (p

U2 - 10.1016/j.jviromet.2012.11.003

DO - 10.1016/j.jviromet.2012.11.003

M3 - Article

C2 - 23183143

SN - 1879-0984

VL - 189

SP - 228

EP - 231

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 1

ER -

A time-resolved immunoassay to measure serum antibodies to the rotavirus VP6 capsid protein

Abstract

Bibliographical note

UN SDGs

Access to Document

Fingerprint

Cite this