A time-resolved immunoassay to measure serum antibodies to the rotavirus VP6 capsid protein

Owen Kavanagh, Xi-Lei Zeng, Sasirekha Ramani, Indrani Mukhopadhya, Sue E Crawford, Gagandeep Kang, Mary K Estes

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The rotavirus (RV) inner capsid protein VP6 is widely used to evaluate immune response during natural infection and in vaccine studies. Recombinant VP6 from the most prevalent circulating rotavirus strains in each subgroup (SG) identified in a birth cohort of children in southern India [SGII (G1P[8]) and SGI (G10P[11])] were produced. The purified proteins were used to measure VP6-specific antibodies in a Dissociation-Enhanced Lanthanide Fluorometric Immunoassay (DELFIA). The ability of the assay to detect a =2 fold rise in IgG level in a panel of serum samples from a longitudinal study was compared to a gold standard virus-capture ELISA. A strong association was observed between the assays (p
Original languageEnglish
Pages (from-to)228-31
JournalJournal of Virological Methods
Volume189
Issue number1
Early online date23 Nov 2012
DOIs
Publication statusPublished - Apr 2013

Bibliographical note

Copyright © 2012 Elsevier B.V. All rights reserved.

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