A transposase-independent mechanism gives rise to precise excision of IS256 from insertion sites in Staphylococcus epidermidis.

Wilma Ziebuhr

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The mobile element IS256 causes phase variation of biofilm formation in Staphylococcus epidermidis by insertion and precise excision from the icaADBC operon. Precise excision, i.e., removal of the target site duplications (TSDs) and restoration of the original DNA sequence, occurs rarely but independently of functional transposase. Instead, the integrity of the TSDs is crucial for precise excision. Excision increased significantly when the TSDs were brought into closer spatial proximity, suggesting that excision is a host-driven process that might involve most likely illegitimate recombination.
Original languageEnglish
Pages (from-to)1488-1490
Number of pages3
JournalJournal of Bacteriology
Volume190(4)
Issue number4
DOIs
Publication statusPublished - Feb 2008

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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