Abnormal endothelial tight junctions in active lesions and normal-appearing white matter in multiple sclerosis

J. Plumb, Stephen McQuaid, Meenakshi Mirakhur, John Kirk

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

Blood-brain barrier (BBB) breakdown, demonstrable in vivo by enhanced MRI is characteristic of new and expanding inflammatory lesions in relapsing remitting and chronic progressive multiple sclerosis (MS). Subtle leakage may also occur in primary progressive MS. However, the anatomical route(s) of BBB leakage have not been demonstrated. We investigated the possible involvement of interendothelial tight junctions (TJ) by examining the expression of TJ proteins (occludin and ZO-1 ) in blood vessels in active MS lesions from 8 cases of MS and in normal-appearing white (NAWM) matter from 6 cases. Blood vessels (10-50 per frozen section) were scanned using confocal laser scanning microscopy to acquire datasets for analysis. TJ abnormalities manifested as beading, interruption, absence or diffuse cytoplasmic localization of fluorescence, or separation of junctions (putative opening) were frequent (affecting 40% of vessels) in oil red-O-positive active plaques but less frequent in NAWM (15%), and in normal (
Original languageEnglish
Pages (from-to)154-169
Number of pages16
JournalBrain Pathology
Volume12 (2)
Issue number2
DOIs
Publication statusPublished - Apr 2002

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)

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