Absolute Quantification of Toxicological Biomarkers by Multiple Reaction Monitoring: Drug Safety Evaluation

Thomas Y. K. Lau, Ben C. Collins, Peter Stone, Ning Tang, William M. Gallagher, Stephen R. Pennington

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

With the advent of “–omics” technologies, there has been an explosion of data generation in the field of toxicology, as well as in many others. As new candidate biomarkers of toxicity are being regularly discovered, the next challenge is to validate these observations in a targeted manner. Traditionally, these validation experiments have been conducted using antibody-based technologies such as Western blotting, ELISA, and immunohistochemistry. However, this often produces a significant bottleneck as the time, cost, and development of successful antibodies are often far outpaced by the generation of targets of interest. In response to this, recently there have been several developments in the use of triple quadrupole (QQQ) mass spectrometry (MS) as a platform to provide quantification of proteins by multiple reaction monitoring (MRM). This technology does not require antibodies; it is typically less expensive and quicker to develop, and has the opportunity for more accessible multiplexing. The speed of these experiments combined with their flexibility and ability to multiplex assays makes the technique a valuable strategy to validate biomarker discovery.
Original languageEnglish
Title of host publicationDrug Safety Evaluation
EditorsJean-Charles Gautier
PublisherHumana Press
Pages417-427
Number of pages11
ISBN (Print)978-1-60327-186-8 978-1-60761-849-2
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Molecular Biology
Volume691

Keywords

  • Mass Spectrometry
  • Proteomics
  • Quantification
  • Pharmacology/Toxicology
  • Laboratory Medicine
  • Catalase
  • Multiple reaction monitoring
  • Biomarkers

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