Absolute quantification of toxicological biomarkers via mass spectrometry

Thomas Y.K. Lau*, Ben C. Collins, Peter Stone, Ning Tang, William M. Gallagher, Stephen R. Pennington

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)


With the advent of “–omics” technologies there has been an explosion of data generation in the field of toxicology, as well as many others. As new candidate biomarkers of toxicity are being regularly discovered, the next challenge is to validate these observations in a targeted manner. Traditionally, these validation experiments have been conducted using antibody-based technologies such as Western blotting, ELISA, and immunohistochemistry. However, this often produces a significant bottleneck as the time, cost, and development of successful antibodies are often far outpaced by the generation of targets of interest. In response to this, there recently have been several developments in the use of triple quadrupole (QQQ) mass spectrometry (MS) as a platform to provide quantification of proteins. This technology does not require antibodies; it is typically less expensive and quicker to develop assays and has the opportunity for more accessible multiplexing. The speed of these experiments combined with their flexibility and ability to multiplex assays makes the technique a valuable strategy to validate biomarker discovery.

Original languageEnglish
Title of host publicationDrug safety evaluation: methods and protocols
EditorsJean-Charles Gautier
PublisherHumana Press
ISBN (Electronic)9781493971725
ISBN (Print)9781493971701, 9781493984060
Publication statusPublished - 27 Jul 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Biomarkers
  • Catalase
  • Mass spectrometry
  • Proteomics
  • Quantification

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


Dive into the research topics of 'Absolute quantification of toxicological biomarkers via mass spectrometry'. Together they form a unique fingerprint.

Cite this