@inbook{4af25f886c5d4f86965a1ad66c548bd2,
title = "Activation of the Non-canonical Inflammasome in Mouse and Human Cells",
abstract = "The non-canonical inflammasome is a signaling platform that allows for the detection of cytoplasmic lipopolysaccharides (LPS) in immune and non-immune cells. Upon detection of LPS, this inflammasome activates the signaling proteases caspase-4 and -5 (in humans) and caspase-11 (in mice). Inflammatory caspases activation leads to caspase self-processing and the cleavage of the pore-forming protein Gasdermin D (GSDMD). GSDMD N-terminal fragments oligomerize and form pores at the plasma membranes, leading to an inflammatory form of cell death called pyroptosis. Here, we describe a simple method to activate the non-canonical inflammasome in myeloid and epithelial cells and to measure its activity using cell death assay and immunoblotting. ",
keywords = "Humans, Caspase, Caspase-4, Gasdermin D, Epithelial cells, Macrophages, Phosphate-Binding Proteins, Lactate dehydrogenase, Pyroptosis, Intracellular Signaling Peptides and Proteins - metabolism, Lipopolysaccharides, Inflammasomes - metabolism, Monocytes, Mice, Neoplasm Proteins - metabolism, Caspase-11, Non-canonical inflammasome, Animals",
author = "Bezbradica, {Jelena S} and Coll, {Rebecca C} and Dave Boucher",
year = "2022",
month = jan,
day = "1",
doi = "10.1007/978-1-0716-2144-8_5",
language = "English",
isbn = "978-1-0716-2143-1",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer",
pages = "51--63",
editor = "Abdul-Sater, {Ali A. }",
booktitle = "The Inflammasome: Methods and Protocols",
}