Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact

Chakkiath Paul Antony, Deepak Kumaresan, Lucia Ferrando, Rich Boden, Hélène Moussard, Ana Fernández Scavino, Yogesh S. Shouche, J Colin Murrell

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

Lonar Lake is a unique saline and alkaline ecosystem formed by meteor impact in the Deccan basalts in India around 52 000 years ago. To investigate the role of methylotrophy in the cycling of carbon in this unusual environment, stable-isotope probing (SIP) was carried out using the one-carbon compounds methane, methanol and methylamine. Denaturing gradient gel electrophoresis fingerprinting analyses performed with heavy 13 C-labelled DNA retrieved from sediment microcosms confirmed the enrichment and labelling of active methylotrophic communities. Clone libraries were constructed using PCR primers targeting 16S rRNA genes and functional genes. Methylomicrobium, Methylophaga and Bacillus spp. were identified as the predominant active methylotrophs in methane, methanol and methylamine SIP microcosms, respectively. Absence of mauA gene amplification in the methylamine SIP heavy fraction also indicated that methylamine metabolism in Lonar Lake sediments may not be mediated by the methylamine dehydrogenase enzyme pathway. Many gene sequences retrieved in this study were not affiliated with extant methanotrophs or methylotrophs. These sequences may represent hitherto uncharacterized novel methylotrophs or heterotrophic organisms that may have been cross-feeding on methylotrophic metabolites or biomass. This study represents an essential first step towards understanding the relevance of methylotrophy in the soda lake sediments of an unusual impact crater structure.

Original languageEnglish
Pages (from-to)1470-1480
Number of pages11
JournalThe ISME Journal
Volume4
Issue number11
DOIs
Publication statusPublished - Nov 2010
Externally publishedYes

Keywords

  • C compounds
  • Lonar Lake
  • Methylomicrobium
  • Methylophaga
  • soda lake
  • stable-isotope probing

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Fingerprint

Dive into the research topics of 'Active methylotrophs in the sediments of Lonar Lake, a saline and alkaline ecosystem formed by meteor impact'. Together they form a unique fingerprint.

Cite this