Activity against Mycobacterium smegmatis and M. tuberculosis by extract of South African medicinal plants

S.P.N. Mativandiela, J.J.M. Meyer, A.A. Hussein, P.J. Houghton, Chris Hamilton, N. Lall

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Seven ethnobotanically selected medicinal plants were screened for their antimycobacterial activity. The mininium inhibitory concentration (MIC) of four plants namely Artemisia afra, Dodonea angustifolia, Drosera capensis and Galenia africana ranged from 0.781 to 6.25 mg/mL against Mycobacterium smegmatis. G. africana showed the best activity exhibiting an MIC of 0.78 mg/mL and a minimum bactericidal concentration (MBC) of 1.56 mg/mL. The MICs of ethanol extracts of A angustifolia and G. africana against M. tuberculosis were found to be 5.0 and 1.2 mg/mL respectively. The mammalian cytotoxicity IC50 value of the most active antimycobacterial extract, from G. africana, was found to be 101.3 mu g/mL against monkey kidney Vero cells. Since the ethanol G. africana displayed the best antimycobacterial activity, it was subjected to fractionation which led to the isolation of a flavone, 5,7,2'-trihydroxyflavone. The MIC of this compound was found to be 0.031 mg/mL against M. smegmatis and 0.10 mg/mL against M. tuberculosis. This study gives some scientific basis to the 14 traditional use of these plants for TB-related symptoms. Copyright (C) 2008 John Wiley & Sons, Ltd.
Original languageEnglish
Pages (from-to)841-845
Number of pages5
JournalPhytotherapy Research
Volume22 (6)
Issue number6
DOIs
Publication statusPublished - Apr 2008

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Mycobacterium smegmatis
flavone
Medicinal Plants
Drosera
Tuberculosis
Ethanol
Artemisia
Vero Cells
Cytotoxicity
Fractionation
Inhibitory Concentration 50
Haplorhini
Kidney
5,7,2'-trihydroxyflavone

Cite this

Mativandiela, S. P. N., Meyer, J. J. M., Hussein, A. A., Houghton, P. J., Hamilton, C., & Lall, N. (2008). Activity against Mycobacterium smegmatis and M. tuberculosis by extract of South African medicinal plants. Phytotherapy Research, 22 (6)(6), 841-845. https://doi.org/10.1002/ptr.2378
Mativandiela, S.P.N. ; Meyer, J.J.M. ; Hussein, A.A. ; Houghton, P.J. ; Hamilton, Chris ; Lall, N. / Activity against Mycobacterium smegmatis and M. tuberculosis by extract of South African medicinal plants. In: Phytotherapy Research. 2008 ; Vol. 22 (6), No. 6. pp. 841-845.
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Mativandiela, SPN, Meyer, JJM, Hussein, AA, Houghton, PJ, Hamilton, C & Lall, N 2008, 'Activity against Mycobacterium smegmatis and M. tuberculosis by extract of South African medicinal plants', Phytotherapy Research, vol. 22 (6), no. 6, pp. 841-845. https://doi.org/10.1002/ptr.2378

Activity against Mycobacterium smegmatis and M. tuberculosis by extract of South African medicinal plants. / Mativandiela, S.P.N.; Meyer, J.J.M.; Hussein, A.A.; Houghton, P.J.; Hamilton, Chris; Lall, N.

In: Phytotherapy Research, Vol. 22 (6), No. 6, 04.2008, p. 841-845.

Research output: Contribution to journalArticle

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Mativandiela SPN, Meyer JJM, Hussein AA, Houghton PJ, Hamilton C, Lall N. Activity against Mycobacterium smegmatis and M. tuberculosis by extract of South African medicinal plants. Phytotherapy Research. 2008 Apr;22 (6)(6):841-845. https://doi.org/10.1002/ptr.2378