Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration

Francesca Ripanti; Claudia Fasolato; Flavia Mazzarda; Simonetta Palleschi; Marina Ceccarini; Chunchun Li; Margherita Bignami; Enrico Bodo; Steven E J Bell; Filomena Mazzei; Paolo Postorino

doi:10.1021/acs.analchem.1c01049

Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration

Francesca Ripanti, Claudia Fasolato, Flavia Mazzarda, Simonetta Palleschi, Marina Ceccarini, Chunchun Li, Margherita Bignami, Enrico Bodo, Steven E J Bell, Filomena Mazzei, Paolo Postorino

School of Chemistry and Chemical Engineering

Research output: Contribution to journal › Article › peer-review

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Abstract

DNA/RNA synthesis precursors are especially vulnerable to damage induced by reactive oxygen species occurring following oxidative stress. Guanosine triphosphates are the prevalent oxidized nucleotides, which can be misincorporated during replication, leading to mutations and cell death. Here, we present a novel method based on micro-Raman spectroscopy, combined with calculations, for the identification, detection, and quantification of oxidized nucleotides at low concentration. We also show that the Raman signature in the terahertz spectral range (

Original language	English
Pages (from-to)	10825-10833
Journal	Analytical chemistry
Volume	93
Issue number	31
Early online date	29 Jul 2021
DOIs	https://doi.org/10.1021/acs.analchem.1c01049
Publication status	Published - 10 Aug 2021

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10.1021/acs.analchem.1c01049Licence: CC BY

Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration
Copyright © 2021 The Authors. Published by American Chemical Society. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 1.96 MBLicence: CC BY

Probing molecular adsorption on nobel metal nanosurfaces with surface-enhanced raman spectroscopy
Author: Li, C., Jul 2022
Supervisor: Bell, S. (Supervisor) & Nockemann, P. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy

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Ripanti, F., Fasolato, C., Mazzarda, F., Palleschi, S., Ceccarini, M., Li, C., Bignami, M., Bodo, E., Bell, S. E. J., Mazzei, F., & Postorino, P. (2021). Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration. Analytical chemistry, 93(31), 10825-10833. https://doi.org/10.1021/acs.analchem.1c01049

Ripanti, Francesca ; Fasolato, Claudia ; Mazzarda, Flavia ; Palleschi, Simonetta ; Ceccarini, Marina ; Li, Chunchun ; Bignami, Margherita ; Bodo, Enrico ; Bell, Steven E J ; Mazzei, Filomena ; Postorino, Paolo. / Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration. In: Analytical chemistry. 2021 ; Vol. 93, No. 31. pp. 10825-10833.

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title = "Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration",

abstract = "DNA/RNA synthesis precursors are especially vulnerable to damage induced by reactive oxygen species occurring following oxidative stress. Guanosine triphosphates are the prevalent oxidized nucleotides, which can be misincorporated during replication, leading to mutations and cell death. Here, we present a novel method based on micro-Raman spectroscopy, combined with calculations, for the identification, detection, and quantification of oxidized nucleotides at low concentration. We also show that the Raman signature in the terahertz spectral range (",

author = "Francesca Ripanti and Claudia Fasolato and Flavia Mazzarda and Simonetta Palleschi and Marina Ceccarini and Chunchun Li and Margherita Bignami and Enrico Bodo and Bell, {Steven E J} and Filomena Mazzei and Paolo Postorino",

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doi = "10.1021/acs.analchem.1c01049",

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Ripanti, F, Fasolato, C, Mazzarda, F, Palleschi, S, Ceccarini, M, Li, C, Bignami, M, Bodo, E, Bell, SEJ, Mazzei, F & Postorino, P 2021, 'Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration', Analytical chemistry, vol. 93, no. 31, pp. 10825-10833. https://doi.org/10.1021/acs.analchem.1c01049

Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration. / Ripanti, Francesca; Fasolato, Claudia; Mazzarda, Flavia; Palleschi, Simonetta; Ceccarini, Marina; Li, Chunchun; Bignami, Margherita; Bodo, Enrico; Bell, Steven E J; Mazzei, Filomena; Postorino, Paolo.

In: Analytical chemistry, Vol. 93, No. 31, 10.08.2021, p. 10825-10833.

Research output: Contribution to journal › Article › peer-review

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T1 - Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration

AU - Ripanti, Francesca

AU - Fasolato, Claudia

AU - Mazzarda, Flavia

AU - Palleschi, Simonetta

AU - Ceccarini, Marina

AU - Li, Chunchun

AU - Bignami, Margherita

AU - Bodo, Enrico

AU - Bell, Steven E J

AU - Mazzei, Filomena

AU - Postorino, Paolo

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AB - DNA/RNA synthesis precursors are especially vulnerable to damage induced by reactive oxygen species occurring following oxidative stress. Guanosine triphosphates are the prevalent oxidized nucleotides, which can be misincorporated during replication, leading to mutations and cell death. Here, we present a novel method based on micro-Raman spectroscopy, combined with calculations, for the identification, detection, and quantification of oxidized nucleotides at low concentration. We also show that the Raman signature in the terahertz spectral range (

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DO - 10.1021/acs.analchem.1c01049

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JO - Analytical Chemistry

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SN - 0003-2700

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Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration

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Student Theses

Probing molecular adsorption on nobel metal nanosurfaces with surface-enhanced raman spectroscopy

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