TY - JOUR
T1 - Advanced Raman Spectroscopy Detection of Oxidative Damage in Nucleic Acid Bases: Probing Chemical Changes and Intermolecular Interactions in Guanosine at Ultralow Concentration
AU - Ripanti, Francesca
AU - Fasolato, Claudia
AU - Mazzarda, Flavia
AU - Palleschi, Simonetta
AU - Ceccarini, Marina
AU - Li, Chunchun
AU - Bignami, Margherita
AU - Bodo, Enrico
AU - Bell, Steven E J
AU - Mazzei, Filomena
AU - Postorino, Paolo
PY - 2021/8/10
Y1 - 2021/8/10
N2 - DNA/RNA synthesis precursors are especially vulnerable to damage induced by reactive oxygen species occurring following oxidative stress. Guanosine triphosphates are the prevalent oxidized nucleotides, which can be misincorporated during replication, leading to mutations and cell death. Here, we present a novel method based on micro-Raman spectroscopy, combined with calculations, for the identification, detection, and quantification of oxidized nucleotides at low concentration. We also show that the Raman signature in the terahertz spectral range (
AB - DNA/RNA synthesis precursors are especially vulnerable to damage induced by reactive oxygen species occurring following oxidative stress. Guanosine triphosphates are the prevalent oxidized nucleotides, which can be misincorporated during replication, leading to mutations and cell death. Here, we present a novel method based on micro-Raman spectroscopy, combined with calculations, for the identification, detection, and quantification of oxidized nucleotides at low concentration. We also show that the Raman signature in the terahertz spectral range (
U2 - 10.1021/acs.analchem.1c01049
DO - 10.1021/acs.analchem.1c01049
M3 - Article
C2 - 34324303
VL - 93
SP - 10825
EP - 10833
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 31
ER -