Allele resolution of HLA-A using oligonucleotide probes in a two-stage typing strategy

F Williams, A Meenagh, A P Maxwell, D Middleton

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

High-resolution polymerase chain reaction using sequence-specific oligonucleotide probes (PCR-SSOP) typing methods for HLA-A identification have been established. The four systems, which operate independently of each other, are intended for use as secondary typing systems following HLA-A identification with a medium-resolution PCR-SSOP technique. The systems, all using digoxigenin-labelled probes, are based on group specific amplifications for resolution of: i) HLA-A*29 & -A*33; ii) HLA-A*24 & -A*30; and iii) HLA-A*26, -A*25, -A*11, -A*34, -A*66 and -A*68 alleles, respectively. The fourth system, for the detection of HLA-A*02 alleles, is a modification of a previously reported PCR-SSOP subtyping system. The methods have been applied to individuals from the local bone marrow registry and HLA-A allele frequencies for the Northern Ireland population have been established.
Original languageEnglish
Pages (from-to)59-68
Number of pages10
JournalTissue Antigens
Volume54
Issue number1
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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